C57BL/6N-Tg(Stab2-icre)1.2Cger/Ieg
| Status | Available to order |
| EMMA ID | EM:15192 |
| International strain name | C57BL/6N-Tg(Stab2-icre)1.2Cger/Ieg |
| Alternative name | C57BL/6N-Tg(Stab2-icre)1.2Cger |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Stab2-icre)1.2Cger |
| Gene/Transgene symbol | Tg(Stab2-icre)1.2Cger |
Information from provider
| Provider | Cyrill Géraud |
| Provider affiliation | Medical Faculty Mannheim University Heidelberg |
| Genetic information | A cDNA containing a codon improved version of cre recombinase followed by polyA signal sequence was fused into BAC clone at exon 1 of mouse Stab2 gene so that cre expression is driven by the regulatory sequences of the BAC gene. The resulting modified BAC was used to generate this transgene. Three founder lines 1.1, 1.2, and 1.3 were identified. The line 1.2 displays cre activity in liver sinusoidal endothelial cells. The genomic insertion is unknown. |
| Phenotypic information | Homozygous:Not applicable, mice strain kept only hemizygous.Heterozygous:The transgenic mouse strain Tg(Stab2-icre)1.2Cger is a cre recombinase driver; cre is expressed in sinusoidal endothelial cells. |
| Breeding history | Transgenic founder mouse was kept in continuous crosses (>10) with C57BL/6N. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6N |
Literature references
- GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.;Géraud Cyrill, Koch Philipp-Sebastian, Zierow Johanna, Klapproth Kay, Busch Katrin, Olsavszky Victor, Leibing Thomas, Demory Alexandra, Ulbrich Friederike, Diett Miriam, Singh Sandhya, Sticht Carsten, Breitkopf-Heinlein Katja, Richter Karsten, Karppinen Sanna-Maria, Pihlajaniemi Taina, Arnold Bernd, Rodewald Hans-Reimer, Augustin Hellmut G, Schledzewski Kai, Goerdt Sergij, ;2017;The Journal of clinical investigation;127;1099-1114; 28218627
- Angiocrine Bmp2 signaling in murine liver controls normal iron homeostasis.;Koch Philipp-Sebastian, Olsavszky Victor, Ulbrich Friederike, Sticht Carsten, Demory Alexandra, Leibing Thomas, Henzler Thomas, Meyer Mathias, Zierow Johanna, Schneider Sven, Breitkopf-Heinlein Katja, Gaitantzi Haristi, Spencer-Dene Bradley, Arnold Bernd, Klapproth Kay, Schledzewski Kai, Goerdt Sergij, Géraud Cyrill, ;2017;Blood;129;415-419; 27903529