Sprague Dawley rat GR+/em4
| Status | Available to order |
| EMMA ID | EM:15187 |
| International strain name | Sprague Dawley rat GR+/em4 |
| Alternative name | Sprague Dawley rat GR+/em4 |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Nr3c1 |
| Gene/Transgene symbol | Nr3c1 |
Information from provider
| Provider | Edith Hummler |
| Provider affiliation | Department of Biomedical Sciences, UNIL |
| Genetic information | In frame deletion at level of the DNA dimerization domain of the rat glucocorticoid receptor (GR) gene. This mutation was described in Ponce de León et al. 2014 as the 5.5, -309 bp deletion and consists of the deletion of the whole second zinc finger domain (G470-E508). The purpose of this line is to assess the function of GR monomers in vivo. |
| Phenotypic information | Homozygous:lethalHeterozygous:The heterozygous mutant rats are viable and fertile and do not present any major macroscopic defects. GR+/em4 display adrenal gland hyperplasia and glucocorticoid excess similar to glucocorticoid resistance syndrome. |
| Breeding history | generated in 2013, on going |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous rat Sprague-Dawley, wild-type rat Sprague-Dawley Charles River Laboratories |
| Stage of embryos | 2-cell |
Literature references
- Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.;Ponce de León Verónica, Mérillat Anne-Marie, Tesson Laurent, Anegón Ignacio, Hummler Edith, ;2014;PloS one;9;e88146; 24523878