Sprague Dawley rat GR+/em3
| Status | Available to order |
| EMMA ID | EM:15186 |
| Citation information | EM:15186 |
| International strain name | Sprague Dawley rat GR+/em3 |
| Alternative name | Sprague Dawley rat GR+/em3 |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | rat Nr3c1em3 |
| Gene/Transgene symbol | rat Nr3c1 |
Information from provider
| Provider | Edith Hummler |
| Provider affiliation | Department of Biomedical Sciences, UNIL |
| Genetic information | In frame deletion at level of the DNA dimerization domain of the rat glucocorticoid receptor (GR) gene. This mutation was described in Ponce de León et al. 2014 as the 6.1, -18 bp deletion and consists of the deletion of ammino acids L475-N480 at the level of the second zinc finger domain. The purpose of this line is to assess the function of GR monomers in vivo. |
| Phenotypic information | Homozygous:lethalHeterozygous:adrenal gland hyperplasia and glucocorticoid excess similar to glucocorticoid resistance syndrome |
| Breeding history | generated in 2013, on going |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous rat Sprague-Dawley males, wild-type rat Sprague-Dawley females |
| Stage of embryos | 2-cell |
Literature references
- Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.;Ponce de León Verónica, Mérillat Anne-Marie, Tesson Laurent, Anegón Ignacio, Hummler Edith, ;2014;PloS one;9;e88146; 24523878