- preweaning lethality, complete penetrance / IMPC
- increased neutrophil cell number / IMPC
- abnormal embryo size / IMPC
- enlarged lymph nodes / IMPC
- decreased lymphocyte cell number / IMPC
- embryonic growth retardation / IMPC
- embryonic lethality prior to tooth bud stage / IMPC
- decreased locomotor activity / IMPC
B6J.Cg-Pik3c2atm1Ehi/Cnrm
Status | Available to order |
EMMA ID | EM:15139 |
International strain name | B6J.Cg-Pik3c2atm1Ehi/Cnrm |
Alternative name | PI3KC2A-LacZ |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Pik3c2atm1Ehi |
Gene/Transgene symbol | Pik3c2a |
Information from provider
Provider | Emilio Hirsch |
Provider affiliation | University of Torino |
Genetic information | A lacZ (bacterial beta-galactosidase)-neoR cassette was inserted in-frame with the ATG start codon of Pik3c2a via bacterial recombination. Constructs were electroporated in embryonic stem cells and chimeras obtained by standard procedures. |
Phenotypic information | Homozygous:Loss of the Pik3c2a gene in mouse has been reported to cause early embryonic lethality, initially ascribed to defective vasculogenesis (Yoshioka et al., Nat. Med., 2012, 18:1560–1569).Heterozygous:Heterozygous mice do not display abnormalities and are viable and fertile. |
Breeding history | Mice were backcrossed for 8 generations to the C57BL/6J background. |
References |
|
Homozygous fertile | no |
Homozygous viable | no |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
IMPC phenotypes (gene matching)
Literature references
- PI3K class II α controls spatially restricted endosomal PtdIns3P and Rab11 activation to promote primary cilium function.;Franco Irene, Gulluni Federico, Campa Carlo C, Costa Carlotta, Margaria Jean Piero, Ciraolo Elisa, Martini Miriam, Monteyne Daniel, De Luca Elisa, Germena Giulia, Posor York, Maffucci Tania, Marengo Stefano, Haucke Volker, Falasca Marco, Perez-Morga David, Boletta Alessandra, Merlo Giorgio R, Hirsch Emilio, ;2014;Developmental cell;28;647-58; 24697898
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