B6J.Cg-Pik3c2atm1Ehi/Cnrm

Status

Available to order

EMMA IDEM:15139
International strain nameB6J.Cg-Pik3c2atm1Ehi/Cnrm
Alternative namePI3KC2A-LacZ
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPik3c2atm1Ehi
Gene/Transgene symbolPik3c2a

Information from provider

ProviderEmilio Hirsch
Provider affiliationUniversity of Torino
Genetic informationA lacZ (bacterial beta-galactosidase)-neoR cassette was inserted in-frame with the ATG start codon of Pik3c2a via bacterial recombination. Constructs were electroporated in embryonic stem cells and chimeras obtained by standard procedures.
Phenotypic informationHomozygous:
Loss of the Pik3c2a gene in mouse has been reported to cause early embryonic lethality, initially ascribed to defective vasculogenesis (Yoshioka et al., Nat. Med., 2012, 18:1560–1569).

Heterozygous:
Heterozygous mice do not display abnormalities and are viable and fertile.
Breeding historyMice were backcrossed for 8 generations to the C57BL/6J background.
References
  • PI3K class II α controls spatially restricted endosomal PtdIns3P and Rab11 activation to promote primary cilium function.;Franco Irene, Gulluni Federico, Campa Carlo C, Costa Carlotta, Margaria Jean Piero, Ciraolo Elisa, Martini Miriam, Monteyne Daniel, De Luca Elisa, Germena Giulia, Posor York, Maffucci Tania, Marengo Stefano, Haucke Volker, Falasca Marco, Perez-Morga David, Boletta Alessandra, Merlo Giorgio R, Hirsch Emilio, ;2014;Developmental cell;28;647-58; 24697898
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (gene matching)
  • preweaning lethality, complete penetrance / IMPC
  • increased neutrophil cell number / IMPC
  • abnormal embryo size / IMPC
  • enlarged lymph nodes / IMPC
  • decreased lymphocyte cell number / IMPC
  • embryonic growth retardation / IMPC
  • embryonic lethality prior to tooth bud stage / IMPC
  • decreased locomotor activity / IMPC

Literature references

  • PI3K class II α controls spatially restricted endosomal PtdIns3P and Rab11 activation to promote primary cilium function.;Franco Irene, Gulluni Federico, Campa Carlo C, Costa Carlotta, Margaria Jean Piero, Ciraolo Elisa, Martini Miriam, Monteyne Daniel, De Luca Elisa, Germena Giulia, Posor York, Maffucci Tania, Marengo Stefano, Haucke Volker, Falasca Marco, Perez-Morga David, Boletta Alessandra, Merlo Giorgio R, Hirsch Emilio, ;2014;Developmental cell;28;647-58; 24697898

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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