B6J;B6N-Nkx1-2tm2296.1(cre/ERT2)Arte/H
| Status | Available to order |
| EMMA ID | EM:15076 |
| Citation information | RRID:IMSR_EM:15076 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J;B6N-Nkx1-2tm2296.1(cre/ERT2)Arte/H |
| Alternative name | Nkx1-2CreERT2 |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Nkx1-2tm2296.1(cre/ERT2)Arte |
| Gene/Transgene symbol | Nkx1-2 |
Information from provider
| Provider | James Briscoe |
| Provider affiliation | The Francis Crick Institute |
| Genetic information | In this mouse line creERT2 recombinase expression is driven under the control of the endogenous promoter of the transcription factor Nkx1-2. The targeting strategy was designed to replace Nkx1-2 exon 1 and the splice donor site at the junction between exon 1 and intron 1 with a cassette containing the open reading frame of creERT2 (see Rodrigo-Albors et al. Development, 2018, 145, dev164319; doi:10.1242/dev.164319). |
| Phenotypic information | Homozygous:creERT2 will be expressed from the endogenous Nkx1-2 promoter and should thus recapitulate the expression pattern of the Nkx1-2 gene. The replacement of the Nkx1-2 coding sequence in exon 1 with the creERT2 and the polyadenylation signal should result in the loss of function of the Nkx1-2 gene. However, loss of function of the Nkx1-2 (aka Sax1) gene did not generate a phenotype in either heterozygous or homozygous mice. This is likely due to genetic compensation or functional redundancy by another related gene/s and confirms previous findings, see Schubert, F. R., Fainsod, A., Gruenbaum, Y. and Gruss, P., 1995, Expression of the novel murine homeobox gene Sax-1 in the developing nervous system, Mech. Dev. 51, 99-114.Heterozygous:Consistent with the Schubert et al. 1995 study; no phenotype has been observed with this reporter line. |
| Breeding history | Taconic provided two breeding pairs of heterozygous C57BL/6–Nkx1-2tm2296(Cre-ER(T2))Arte mice. These mice carried a puromycin-expressing cassette flanked by F3 sites, which was removed upon crossing to flp recombinase-expressing mice. The resulting mice express creERT2 from the endogenous Nkx1-2 promoter (Nkx1-2creERT2). Nkx1-2CreERT2 mice were then bred to homozygosity to establish a breeding colony. We maintained this homozygous Nkx1-2CreERT2 colony for more than nine generations without any obvious deleterious effects. Genetic background: C57BL/6NTac and C57BL/6J mixed. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions.;Rodrigo Albors Aida, Halley Pamela A, Storey Kate G, ;2018;Development (Cambridge, England);145;; 30201686