C57BL/6NCrl-Tg(Clec4g-icre)1.1Sgoe/Ieg
| Status | Available to order |
| EMMA ID | EM:15074 |
| International strain name | C57BL/6NCrl-Tg(Clec4g-icre)1.1Sgoe/Ieg |
| Alternative name | Tg(Clec4g-icre)1.1Sgoe |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Clec4g-icre)1.1Sgoe |
| Gene/Transgene symbol | Tg(Clec4g-icre)1.1Sgoe |
Information from provider
| Provider | Sergij Goerdt |
| Provider affiliation | Dermatology, Medical Faculty Mannheim University Heidelberg |
| Genetic information | A cDNA containing a codon improved version of the cre recombinase followed by a polyA signal sequence was fused in frame into a BAC clone (RPCI-23-239-A7) at exon 1 of mouse Clec4g so that cre expression is driven by the regulatory sequences of the BAC gene. The resulting modified BAC was used to generate this transgenic mouse strain. Three founder lines 1.1, 1.2 and 1.3 were identified. Founder 1.1 was used in experiments. The genomic insertion is unknown. |
| Phenotypic information | Homozygous:Not applicable, the mouse strain is kept only hemizygous.Heterozygous:The transgenic mouse strain Tg(Clec4g-icre)1.1Sgoe is a cre driver mouse; cre recombinase is expressed in sinusoidal endothelial cells. |
| Breeding history | Transgenic founder mouse was then crossed to the C57BL/6NCrl genetic background (> than 10 backcrosses). |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6NCrl |
Literature references
- Hepatic Endothelial Notch Activation Protects against Liver Metastasis by Regulating Endothelial-Tumor Cell Adhesion Independent of Angiocrine Signaling.;Wohlfeil Sebastian A, Häfele Verena, Dietsch Bianca, Schledzewski Kai, Winkler Manuel, Zierow Johanna, Leibing Thomas, Mohammadi Mona Malek, Heineke Joerg, Sticht Carsten, Olsavszky Victor, Koch Philipp-Sebastian, Géraud Cyrill, Goerdt Sergij, ;2019;Cancer research;79;598-610; 30530502
- Endothelial GATA4 controls liver fibrosis and regeneration by preventing a pathogenic switch in angiocrine signaling.;Winkler Manuel, Staniczek Theresa, Kürschner Sina Wietje, Schmid Christian David, Schönhaber Hiltrud, Cordero Julio, Kessler Linda, Mathes Arthur, Sticht Carsten, Neßling Michelle, Uvarovskii Alexey, Anders Simon, Zhang Xue-Jun, von Figura Guido, Hartmann Daniel, Mogler Carolin, Dobreva Gergana, Schledzewski Kai, Géraud Cyrill, Koch Philipp-Sebastian, Goerdt Sergij, ;2021;Journal of hepatology;74;380-393; 32916216