B6J.Cg-Gpr37l1tm1.2Cnrm/Cnrm
| Status | Available to order |
| EMMA ID | EM:15003 |
| International strain name | B6J.Cg-Gpr37l1tm1.2Cnrm/Cnrm |
| Alternative name | Gpr37l1-KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gpr37l1tm1.2-KO-Cnrm |
| Gene/Transgene symbol | Gpr37l1 |
Information from provider
| Provider | Daniela Marazziti |
| Provider affiliation | Istitute of Biochemistry and Cell Biology (IBBC), Consiglio Nazionale delle Ricerche (CNR) |
| Genetic information | Single loxP site inserted upstream of Gpr37l1 exon 1 and floxed neomycin-resistance cassette inserted in intron 1 by homologous recombination in ES cells. A targeted XSV1 (129S6/SvEvTac) ES cell clone was used to produce the Gpr37l1-floxed/neo mouse mutant strain (EMMA strain ID EM:15002). Heterozygous Gpr37l1-floxed/neo males were crossed to Tg(CMV-cre)1Cgn cre-deleter females (from a C57BL/6J-backrossed strain, EMMA strain ID EM:01135). In vivo expression of cre recombinase resulted in the excision of the floxed neo cassette and exon 1 and the Tg(CMV-cre)1Cgn transgene was then bred out. |
| Phenotypic information | GPR37L1 is a 7-transmembrane span protein, highly homologous to GPR37, a parkin-associated endothelin B receptor-like protein. Mammalian GPR37L1 receptors are highly expressed in various brain regions and have been reported to interact with the prosaposin glycoprotein, as well as its derived, cytoprotective peptides and analogues. In mouse cerebellar astrocytes the GPR37L1 protein is associated to patched 1 (Ptch1), the primary cilium's sonic hedgehog (Shh) coreceptor, and to megalencephalic leukoencephalopathy membrane proteins. Homozygous Gpr37l1-KO mice present with precocious cerebellum development and improved motor functions at juvenile and adult age. Cerebellar primary astrocytes from homoz. Gpr37l1-KO mice exhibit altered Ptch1 intracellular trafficking, as well as strongly increased Shh production and Shh-induced proliferation. Gpr37l1/Ptch1 double-mutant mice (heteroz. Ptch1-KO, EMMA strain ID EM:00159, and homoz. Gpr37l1-KO), show a marked delay in Shh-type medulloblastoma occurrence and decreased incidence of pre-tumorigenic lesions. |
| Breeding history | Backcrossed to C57BL/6J, N=9 |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Genetic ablation of Gpr37l1 delays tumor occurrence in Ptch1+/- mouse models of medulloblastoma.;Di Pietro Chiara, La Sala Gina, Matteoni Rafaele, Marazziti Daniela, Tocchini-Valentini Glauco P, ;2019;Experimental neurology;312;33-42; 30452905
- Primary Cilia in the Murine Cerebellum and in Mutant Models of Medulloblastoma.;Di Pietro Chiara, Marazziti Daniela, La Sala Gina, Abbaszadeh Zeinab, Golini Elisabetta, Matteoni Rafaele, Tocchini-Valentini Glauco P, ;2017;Cellular and molecular neurobiology;37;145-154; 26935062
- Precocious cerebellum development and improved motor functions in mice lacking the astrocyte cilium-, patched 1-associated Gpr37l1 receptor.;Marazziti Daniela, Di Pietro Chiara, Golini Elisabetta, Mandillo Silvia, La Sala Gina, Matteoni Rafaele, Tocchini-Valentini Glauco P, ;2013;Proceedings of the National Academy of Sciences of the United States of America;110;16486-91; 24062445
- Gpr37l1/prosaposin receptor regulates Ptch1 trafficking, Shh production, and cell proliferation in cerebellar primary astrocytes.;La Sala Gina, Di Pietro Chiara, Matteoni Rafaele, Bolasco Giulia, Marazziti Daniela, Tocchini-Valentini Glauco P, ;2020;Journal of neuroscience research;0;1649-1665; 33350496
- Identification of the GlialCAM interactome: the G protein-coupled receptors GPRC5B and GPR37L1 modulate megalencephalic leukoencephalopathy proteins.;Alonso-Gardón Marta, Elorza-Vidal Xabier, Castellanos Aida, La Sala Gina, Armand-Ugon Mercedes, Gilbert Alice, Di Pietro Chiara, Pla-Casillanis Adrià, Ciruela Francisco, Gasull Xavier, Nunes Virginia, Martínez Albert, Schulte Uwe, Cohen-Salmon Martine, Marazziti Daniela, Estévez Raúl, ;2021;Human molecular genetics;30;; 34100078