B6J.Cg-Gpr37tm1.2Cnrm/Cnrm
| Status | Available to order |
| EMMA ID | EM:15001 |
| International strain name | B6J.Cg-Gpr37tm1.2Cnrm/Cnrm |
| Alternative name | Gpr37-KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gpr37tm2-KO-Cnrm |
| Gene/Transgene symbol | Gpr37 |
Information from provider
| Provider | Daniela Marazziti |
| Provider affiliation | Istitute of Biochemistry and Cell Biology (IBBC), Consiglio Nazionale delle Ricerche (CNR) |
| Genetic information | Single loxP site inserted upstream of Gpr37 exon 1 and floxed neomycin-resistance cassette inserted in intron 1 by homologous recombination in ES cells. In vitro expression of cre recombinase in a targeted E14.1 (129P2/OlaHsd) ES cell clone eliminated the floxed neo cassette and left exon 1 flanked by loxP sites. The ES cell clone was then used to produce the Gpr37-floxed mouse mutant strain (EMMA strain ID EM:15000). Heterozygous Gpr37-floxed males were crossed to Tg(CMV-cre)1Cgn cre-deleter females (from a C57BL/6J-backrossed strain, EMMA strain ID EM:01135). In vivo expression of cre recombinase resulted in the excision of floxed exon 1 and the Tg(CMV-cre)1Cgn transgene was then bred out. |
| Phenotypic information | GPR37 is a 7-transmembrane span protein, partly homologous to endothelin B receptors. It is highly expressed in several regions of human and rodent brain, including striatum's dopaminergic neurons where it can be found associated to parkin. Mammalian GPR37 receptors have been reported to interact with the prosaposin glycoprotein, as well as its derived, cytoprotective peptides and analogues. Homozygous Gpr37-KO mice present with altered dopamine signaling and marked resistance to MPTP, a potent Parkinson's disease-inducing toxin. Gpr37 has been found to interact with the dopamine transporter (DAT) protein at pre-synaptic neuronal membranes and homozygous Gpr37-KO mice show enhanced, DAT-mediated dopamine uptake in striatum's membrane samples. They also manifest decreased cocaine-induced locomotor activity and dopaminergic antagonist-induced catalepsy, as well as age- and gender-affected, non-motor behavioral phenotypes. |
| Breeding history | Backcrossed to C57BL/6J, N=9. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Altered dopamine signaling and MPTP resistance in mice lacking the Parkinson's disease-associated GPR37/parkin-associated endothelin-like receptor.;Marazziti Daniela, Golini Elisabetta, Mandillo Silvia, Magrelli Armando, Witke Walter, Matteoni Rafaele, Tocchini-Valentini Glauco P, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;10189-94; 15218106
- GPR37 associates with the dopamine transporter to modulate dopamine uptake and behavioral responses to dopaminergic drugs.;Marazziti Daniela, Mandillo Silvia, Di Pietro Chiara, Golini Elisabetta, Matteoni Rafaele, Tocchini-Valentini Glauco P, ;2007;Proceedings of the National Academy of Sciences of the United States of America;104;9846-51; 17519329
- Mice lacking the Parkinson's related GPR37/PAEL receptor show non-motor behavioral phenotypes: age and gender effect.;Mandillo S, Golini E, Marazziti D, Di Pietro C, Matteoni R, Tocchini-Valentini G P, ;2013;Genes, brain, and behavior;12;465-77; 23574697