B6N.Cg-Tg(Nes-cre/ERT2)62.33Ics/Ics

Status	Available to order
EMMA ID	EM:14963
International strain name	B6N.Cg-Tg(Nes-cre/ERT2)62.33Ics/Ics
Alternative name	Tg(Nes-creERT2)62.33.Ics
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Nes-cre/ERT2)62.33Ics
Gene/Transgene symbol	Tg(Nes-cre/ERT2)62.33Ics

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	The line was obtained by microinjection of a circular murine BAC transgene (RP23-377C20), previously modified by recombineering at the ATG of the Nes gene of a rabbit-b-globin intron-CreERT2 cassette followed by a flipped PGK-Tn5-Kana/NeoR cassette. The genetic background of the fertilized oocytes was FVB/N. The founder was backcrossed to a C57BL/6J genetic background. The subline 62.33 was analyzed and cryopreserved. The creERT2 expression has been show to be specific. The information available on the characterization of the line, e.g. expression analysis, histology, paper reference, is available on the web page linked here.
Phenotypic information	Homozygous: NA Heterozygous: NA
Breeding history	Backcrossed to C57BL/6N for 7 generations.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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