C57BL/6-Mpc1tm-(tetO)Itl/H
| Status | Available to order |
| EMMA ID | EM:14916 |
| Citation information | RRID:IMSR_EM:14916 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Mpc1tm-(tetO)Itl/H |
| Alternative name | MPC1 tetO KI |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Mpc1-FAST |
| Gene/Transgene symbol | Mpc1 |
Information from provider
| Provider | Philip Eaton |
| Provider affiliation | Queen Mary University |
| Genetic information | Targeted Mpc1 FAST (Flexible Accelerated STOP Tetracycline Operator (tetO) knock-in) mouse line was generated by Ingenious Targeting Laboratory (iTL) technologies (Ronkonkoma, New York). Briefly, vectors that contained a loxP-tetO (PTre3G promoter) sequence were inserted immediately upstream of the endogenous ATG initiation site in exon 1 of the mitochondrial pyruvate carrier 1 (Mpc1) gene sequence. A single gene targeting event yields two distinct knock-in mice: STOP-tetO and tetO knockin that permits generation of multiple strains with variable expression patterns: 1) knockout, 2) cre-mediated rescue, 3) tetracycline-controlled transcriptional activator (tTA)-mediated misexpression, 4) tetracycline-controlled transcriptional activator (tTA)-mediated overexpression, and 5) tetracycline-controlled transcriptional silencer (tTS)-mediated conditional knockout/knockdown. Using the FAST system, multiple gain-of-function and loss-of-function strains can therefore be generated on a time scale not previously achievable. |
| Phenotypic information | Homozygous:Phenotypic effects only shown when crossed to mice carrying the cre/loxP and the tetracycline on/off systems.Heterozygous:Phenotypic effects only shown when crossed to mice carrying the cre/loxP and the tetracycline on/off systems. |
| Breeding history | These mice were backcrossed more than 10 times to C57BL/6NJ over the last 5 years. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Mitochondrial pyruvate carrier abundance mediates pathological cardiac hypertrophy.;Fernandez-Caggiano Mariana, Kamynina Alisa, Francois Asvi A, Prysyazhna Oleksandra, Eykyn Thomas R, Krasemann Susanne, Crespo-Leiro Maria G, Vieites Maria Garcia, Bianchi Katiuscia, Morales Valle, Domenech Nieves, Eaton Philip, ;2020;Nature metabolism;2;1223-1231; 33106688