B6.Cg-Tg(Cdh5-cre/ERT2)1Rha/H

Status	Available to order
EMMA ID	EM:14891
International strain name	B6.Cg-Tg(Cdh5-cre/ERT2)1Rha/H
Alternative name	C57BL/6-Tg(Cdh5-Cre/ERT2)1Rha
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Cdh5-cre/ERT2)1Rha
Gene/Transgene symbol	Tg(Cdh5-cre/ERT2)1Rha

Information from provider

Provider	Ralf Adams
Provider affiliation	Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine
Genetic information	An inducible cre deleter under the control of the vascular endothelial cadherin (Cdh5(PAC)) promoter; cre recombination is induced upon administration of tamoxifen. Useful for the study of angiogenesis, atherosclerosis and neovascularisation. Non-induced Cdh5(PAC)-creERT2 mice show absence of cre recombinase activity, while tamoxifen-induced Cdh5(PAC)-creERT2 mice show high levels of recombinase-mediated gene deletion in vascular endothelial cells (95%+).
Phenotypic information	Homozygous: Not known Heterozygous: Normal appearance
Breeding history	Founder lines were backcrossed to C57BL/6 for at least 10 generations.
References	Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.;Wang Yingdi, Nakayama Masanori, Pitulescu Mara E, Schmidt Tim S, Bochenek Magdalena L, Sakakibara Akira, Adams Susanne, Davy Alice, Deutsch Urban, Lüthi Urs, Barberis Alcide, Benjamin Laura E, Mäkinen Taija, Nobes Catherine D, Adams Ralf H, ;2010;Nature;465;483-6; 20445537 Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.;Pitulescu Mara E, Schmidt Inga, Benedito Rui, Adams Ralf H, ;2010;Nature protocols;5;1518-34; 20725067 Endothelial cells require functional FLVCR1a during developmental and adult angiogenesis.;Petrillo Sara, De Giorgio F, Bertino F, Garello F, Bitonto V, Longo D L, Mercurio S, Ammirata G, Allocco A L, Fiorito V, Chiabrando D, Altruda F, Terreno E, Provero P, Munaron L, Genova T, Nóvoa A, Carlos A R, Cardoso S, Mallo M, Soares M P, Tolosano E, ;2023;Angiogenesis;26;365-384; 36631598 Loss of Jagged1 in mature endothelial cells causes vascular dysfunction with alterations in smooth muscle phenotypes.;Breikaa Randa M, Denman Kimberly, Ueyama Yukie, McCallinhart Patricia E, Khan Aiman Q, Agarwal Gunjan, Trask Aaron J, Garg Vidu, Lilly Brenda, ;2022;Vascular pharmacology;145;107087; 35792302 Desert Hedgehog-Driven Endothelium Integrity Is Enhanced by Gas1 (Growth Arrest-Specific 1) but Negatively Regulated by Cdon (Cell Adhesion Molecule-Related/Downregulated by Oncogenes).;Chapouly Candice, Hollier Pierre-Louis, Guimbal Sarah, Cornuault Lauriane, Gadeau Alain-Pierre, Renault Marie-Ange, ;2020;Arteriosclerosis, thrombosis, and vascular biology;40;e336-e349; 33028094 Exacerbated atherosclerosis in progeria is prevented by progerin elimination in vascular smooth muscle cells but not endothelial cells.;Benedicto Ignacio, Carmona Rosa M, Barettino Ana, Espinós-Estévez Carla, Gonzalo Pilar, Nevado Rosa M, de la Fuente-Pérez Miguel, Andrés-Manzano María J, González-Gómez Cristina, Rolas Loïc, Dorado Beatriz, Nourshargh Sussan, Hamczyk Magda R, Andrés Vicente, ;2024;Proceedings of the National Academy of Sciences of the United States of America;121;e2400752121; 38648484 FLVCR1a Controls Cellular Cholesterol Levels through the Regulation of Heme Biosynthesis and Tricarboxylic Acid Cycle Flux in Endothelial Cells.;Manco Marta, Ammirata Giorgia, Petrillo Sara, De Giorgio Francesco, Fontana Simona, Riganti Chiara, Provero Paolo, Fagoonee Sharmila, Altruda Fiorella, Tolosano Emanuela, ;2024;Biomolecules;14;308-325.e11; 38397386 The phosphodiesterase 2A controls lymphatic junctional maturation via cGMP-dependent notch signaling.;Carlantoni Claudia, Liekfeld Leon M H, Hemkemeyer Sandra A, Schreier Danny, Saygi Ceren, Kurelic Roberta, Cardarelli Silvia, Kalucka Joanna, Schulte Christian, Beerens Manu, Mailer Reiner K, Schäffer Tilman E, Naro Fabio, Pellegrini Manuela, Nikolaev Viacheslav O, Renné Thomas, Frye Maike, ;2024;Developmental cell;59;362-368; 38159569 Whole mount of adult ear skin as a model to study vascular malformations.;Decker-Rockefeller Brandee, Li Qingfen, Pumiglia Kevin, ;2023;Animal models and experimental medicine;6;; 37682010
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.;Wang Yingdi, Nakayama Masanori, Pitulescu Mara E, Schmidt Tim S, Bochenek Magdalena L, Sakakibara Akira, Adams Susanne, Davy Alice, Deutsch Urban, Lüthi Urs, Barberis Alcide, Benjamin Laura E, Mäkinen Taija, Nobes Catherine D, Adams Ralf H, ;2010;Nature;465;483-6; 20445537
Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.;Pitulescu Mara E, Schmidt Inga, Benedito Rui, Adams Ralf H, ;2010;Nature protocols;5;1518-34; 20725067
Endothelial cells require functional FLVCR1a during developmental and adult angiogenesis.;Petrillo Sara, De Giorgio F, Bertino F, Garello F, Bitonto V, Longo D L, Mercurio S, Ammirata G, Allocco A L, Fiorito V, Chiabrando D, Altruda F, Terreno E, Provero P, Munaron L, Genova T, Nóvoa A, Carlos A R, Cardoso S, Mallo M, Soares M P, Tolosano E, ;2023;Angiogenesis;26;365-384; 36631598
Loss of Jagged1 in mature endothelial cells causes vascular dysfunction with alterations in smooth muscle phenotypes.;Breikaa Randa M, Denman Kimberly, Ueyama Yukie, McCallinhart Patricia E, Khan Aiman Q, Agarwal Gunjan, Trask Aaron J, Garg Vidu, Lilly Brenda, ;2022;Vascular pharmacology;145;107087; 35792302
Desert Hedgehog-Driven Endothelium Integrity Is Enhanced by Gas1 (Growth Arrest-Specific 1) but Negatively Regulated by Cdon (Cell Adhesion Molecule-Related/Downregulated by Oncogenes).;Chapouly Candice, Hollier Pierre-Louis, Guimbal Sarah, Cornuault Lauriane, Gadeau Alain-Pierre, Renault Marie-Ange, ;2020;Arteriosclerosis, thrombosis, and vascular biology;40;e336-e349; 33028094
Exacerbated atherosclerosis in progeria is prevented by progerin elimination in vascular smooth muscle cells but not endothelial cells.;Benedicto Ignacio, Carmona Rosa M, Barettino Ana, Espinós-Estévez Carla, Gonzalo Pilar, Nevado Rosa M, de la Fuente-Pérez Miguel, Andrés-Manzano María J, González-Gómez Cristina, Rolas Loïc, Dorado Beatriz, Nourshargh Sussan, Hamczyk Magda R, Andrés Vicente, ;2024;Proceedings of the National Academy of Sciences of the United States of America;121;e2400752121; 38648484
FLVCR1a Controls Cellular Cholesterol Levels through the Regulation of Heme Biosynthesis and Tricarboxylic Acid Cycle Flux in Endothelial Cells.;Manco Marta, Ammirata Giorgia, Petrillo Sara, De Giorgio Francesco, Fontana Simona, Riganti Chiara, Provero Paolo, Fagoonee Sharmila, Altruda Fiorella, Tolosano Emanuela, ;2024;Biomolecules;14;308-325.e11; 38397386
The phosphodiesterase 2A controls lymphatic junctional maturation via cGMP-dependent notch signaling.;Carlantoni Claudia, Liekfeld Leon M H, Hemkemeyer Sandra A, Schreier Danny, Saygi Ceren, Kurelic Roberta, Cardarelli Silvia, Kalucka Joanna, Schulte Christian, Beerens Manu, Mailer Reiner K, Schäffer Tilman E, Naro Fabio, Pellegrini Manuela, Nikolaev Viacheslav O, Renné Thomas, Frye Maike, ;2024;Developmental cell;59;362-368; 38159569
Whole mount of adult ear skin as a model to study vascular malformations.;Decker-Rockefeller Brandee, Li Qingfen, Pumiglia Kevin, ;2023;Animal models and experimental medicine;6;; 37682010

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