IMPC phenotypes (gene matching)
STOCK Daglatm1.2Zim/Orl
| Status | Under development - register interest |
| EMMA ID | EM:14883 |
| International strain name | STOCK Daglatm1.2Zim/Orl |
| Alternative name | B6.cg Dagla tm1.2Zim |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Daglatm1.2Zim |
| Gene/Transgene symbol | Dagla |
Information from provider
| Provider | Andreas Zimmer |
| Provider affiliation | Institut für Molekulare Pychiatrie |
| Genetic information | The murine Dagla gene is located at chromosome 19 and comprises 20 exons. In the Dagla-tm1.2Zim (Dagla knock-out) line the exon 1 was removed by cre recombinase-mediated excision (the floxed strain used is EMMA strain EM:14881). Depiction of the cloning and knock-out strategy is shown at Fig. 1 in Jenniches et al., Biol. Psychiatry, 2015. |
| Phenotypic information | Homozygous:The Dagla-tm1.2Zim homozygous knock-out line is epilepsy prone, spontaneous epileptic seizures are observed especially in stress situation. The body weight of the mice is lower than in wild-type littermates from a heterozygous breeding. The behavioral changes induced by Dagla deletion include a reduced exploration of the central area of the open field, a maternal neglect behavior, a fear extinction deficit, increased behavioral despair, increased anxiety-related behaviors in the light/dark box, and reduced hippocampal neurogenesis. Heterozygous:Heterozygous mice were not tested. |
| Breeding history | 10 generations on C57BL/6J. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased body weight / MGI
- abnormal lipid level / MGI
- absent long term depression / MGI
- abnormal CNS synaptic transmission / MGI
- abnormal excitatory postsynaptic currents / MGI
- abnormal inhibitory postsynaptic currents / MGI
- increased single cell response threshold / MGI
- abnormal nervous system morphology / MGI
- decreased neuronal precursor cell number / MGI
- abnormal neuron proliferation / MGI
Literature references
- Anxiety, Stress, and Fear Response in Mice With Reduced Endocannabinoid Levels.;Jenniches Imke, Ternes Svenja, Albayram Onder, Otte David M, Bach Karsten, Bindila Laura, Michel Kerstin, Lutz Beat, Bilkei-Gorzo Andras, Zimmer Andreas, ;2016;Biological psychiatry;79;858-868; 25981172