Sprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng

Status

Available to order

EMMA IDEM:14799
International strain nameSprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng
Alternative nameSprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng
Strain typeEndonuclease-mediated
Allele/Transgene symbolUnknown at present
Gene/Transgene symbolGnal

Information from provider

ProviderHuu Phuc Nguyen
Provider affiliationInstitute od Medical Genetics and Applied Genomics, University of Tuebingen
Genetic informationCrl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng rats were generated by CRISPR/Cas9 technology. A deletion of 135 base pairs, corresponding to position 44-178, downstream of the translation initiation start ATG of the Gnal splicing variant 2, results in a deletion mutation. CRISPR technology details: self-synthesized Cas9 mRNA using px 330 vector, applying T7 transcription kit followed by Poly-Adenylation and Capping. No sequencing performed, whether Cas9 has been inserted into the rat genome is unknown.
Phenotypic informationHomozygous:
No homozygous was generated

Heterozygous:
No characterization of phenotype
Breeding historyCrl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng is an outbred strain, the status now is about 16 generations.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous rat Sprague-Dawley, wild-type rat Sprague-Dawley
Stage of embryos2-cell

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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