Sprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng
Status | Available to order |
EMMA ID | EM:14799 |
International strain name | Sprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng |
Alternative name | Sprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng |
Strain type | Endonuclease-mediated |
Allele/Transgene symbol | Unknown at present |
Gene/Transgene symbol | Gnal |
Information from provider
Provider | Huu Phuc Nguyen |
Provider affiliation | Institute od Medical Genetics and Applied Genomics, University of Tuebingen |
Genetic information | Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng rats were generated by CRISPR/Cas9 technology. A deletion of 135 base pairs, corresponding to position 44-178, downstream of the translation initiation start ATG of the Gnal splicing variant 2, results in a deletion mutation. CRISPR technology details: self-synthesized Cas9 mRNA using px 330 vector, applying T7 transcription kit followed by Poly-Adenylation and Capping. No sequencing performed, whether Cas9 has been inserted into the rat genome is unknown. |
Phenotypic information | Homozygous:No homozygous was generatedHeterozygous:No characterization of phenotype |
Breeding history | Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng is an outbred strain, the status now is about 16 generations. |
References | None available |
Homozygous fertile | not known |
Homozygous viable | not known |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Animals used for archiving | heterozygous rat Sprague-Dawley, wild-type rat Sprague-Dawley |
Stage of embryos | 2-cell |
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