MGI phenotypes (gene matching)
C57BL/6N-Hmgcrem1Lclem/Kctt
| Status | Available to order |
| EMMA ID | EM:14795 |
| Citation information | RRID:IMSR_EM:14795 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Hmgcrem1Lclem/Kctt |
| Alternative name | C57BL/6N-Hmgcrem1Lclem/Lclem |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Hmgcrem1Lclem |
| Gene/Transgene symbol | Hmgcr |
Information from provider
| Provider | Helgi Schiöth |
| Provider affiliation | Department of Surgical Sciences, Functional Pharmacology and Neuroscience, Uppsala University |
| Genetic information | LoxP sites have been inserted around exon 5 of the Hmgcr gene by CRISPR/Cas9 mediated genome engineering. CRISPR technology details: Cas9 protein (from New England Biolabs) and gRNA were co-injected into fertilized eggs, therefore Cas9 did not integrate in the mouse genome. |
| Phenotypic information | Homozygous:The mice have no pathological phenotype, since the mutation is conditional.Heterozygous:The mice have no pathological phenotype, since the mutation is conditional. |
| Breeding history | Heterozygous founders were bred with C57BL/6NTac wild-type mice to increase the colony. After this, the heterozygous mice were bred to homozygosity and inbred for 4 generations before conservation. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | homozygous males |