IMPC phenotypes (gene matching)
- small superior vagus ganglion / IMPC
RjHan:SD rat CST3
| Status | Available to order |
| EMMA ID | EM:14763 |
| International strain name | RjHan:SD rat CST3 |
| Alternative name | rCST3 |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Zygote electroporation of gene-specific gRNA, Cas9 and ssODN template was used to introduce M88Q point mutation. |
| Gene/Transgene symbol | Cst3 |
Information from provider
| Provider | Jana Kopkanova |
| Provider affiliation | Transgenic and Archiving Unit, Institute of Molecular Genetics of the Czech Academy of Sciences |
| Genetic information | cystatin C (Cst3, CYSC, MGC105556) gene; mutant rats were generated by zygote electroporation of gene-specific gRNA (5´-CTATTATTTGGATGTGGAGA-3´), Cas9 and ssODN (5´-CAGTTAGTCAAATTTGTCTGGGACTTGGTACATGTAGTTCGGCCCtgCTCCA CgTCCAAATAATAGTTTATTCCAGCCACAAGCTACAGTGAAAAAGAG-3´) template was used to introduce M88Q (p.Met88Gln) point mutation into the 2nd coding exon. Positive founders were identified by RFLP analysis. |
| Phenotypic information | Homozygous:not analyzedHeterozygous:not analyzed |
| Breeding history | 2 generations crossed to RjHan:SD background |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
| Animals used for archiving | wild-type rat Sprague-Dawley, heterozygous rat Sprague-Dawley |
| Stage of embryos | 2-cell |