CD1;B6N-Igs32tm2(ACTB-tdTomato,-EGFP)Biat/Biat
| Status | Available to order |
| EMMA ID | EM:14701 |
| International strain name | CD1;B6N-Igs32tm2(ACTB-tdTomato,-EGFP)Biat/Biat |
| Alternative name | CD1;B6N-MADM-TG |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Igs32tm2(ACTB-tdTomato,-EGFP)Biat |
| Gene/Transgene symbol | Igs32 |
Information from provider
| Provider | Simon Hippenmeyer |
| Provider affiliation | Institute of Science and Technology Austria (IST Austria) |
| Genetic information | The TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the "Hipp9" locus on chromosome 9 (between Tmem123 and Birc2). (PMID 34161767) |
| Phenotypic information | Homozygous:not availableHeterozygous:not available |
| Breeding history | Chimeras were generated by injecting C57BL/6N embryonic stem cells into BALB/cRj blastocysts and then crossed with CD1 mice. Afterwards they were intercrossed. |
| References |
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| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Literature references
- A genome-wide library of MADM mice for single-cell genetic mosaic analysis.;Contreras Ximena, Amberg Nicole, Davaatseren Amarbayasgalan, Hansen Andi H, Sonntag Johanna, Andersen Lill, Bernthaler Tina, Streicher Carmen, Heger Anna, Johnson Randy L, Schwarz Lindsay A, Luo Liqun, Rülicke Thomas, Hippenmeyer Simon, ;2021;Cell reports;35;109274; 34161767
- Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance.;Hippenmeyer Simon, Johnson Randy L, Luo Liqun, ;2013;Cell reports;3;960-7; 23453967
- Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration.;Hippenmeyer Simon, Youn Yong Ha, Moon Hyang Mi, Miyamichi Kazunari, Zong Hui, Wynshaw-Boris Anthony, Luo Liqun, ;2010;Neuron;68;695-709; 21092859