CD1;B6N-Igs5^{tm1(ACTB-tdTomato,-EGFP)Luo}/Biat

Status	Available to order
EMMA ID	EM:14697
International strain name	CD1;B6N-Igs5^{tm1(ACTB-tdTomato,-EGFP)Luo}/Biat
Alternative name	CD1;B6N-MADM-TG; Igs5; MADM-7-TG
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Igs5^{tm1(ACTB-tdTomato,-EGFP)Luo}
Gene/Transgene symbol	Igs5

Information from provider

Provider	Simon Hippenmeyer
Provider affiliation	Institute of Science and Technology Austria (IST Austria)
Additional owner	Prof. Liqun Luo, Stanford University, Biology Department, Stanford, USA.
Genetic information	The TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the Iis5 locus locate at about 0.7 kb downstream of exon 5 of the Rps9 gene at 2.13cM on chromosome 7. (PMID 23453967)
Phenotypic information	Homozygous: not available Heterozygous: not available
Breeding history	Chimeras were generated by injecting C57BL/6N embryonic stem cells into BALB/cRj blastocysts and then crossed with CD1 mice. Afterwards they were intercrossed.
References	Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance.;Hippenmeyer Simon, Johnson Randy L, Luo Liqun, ;2013;Cell reports;3;960-7; 23453967 Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration.;Hippenmeyer Simon, Youn Yong Ha, Moon Hyang Mi, Miyamichi Kazunari, Zong Hui, Wynshaw-Boris Anthony, Luo Liqun, ;2010;Neuron;68;695-709; 21092859
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Literature references

Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance.;Hippenmeyer Simon, Johnson Randy L, Luo Liqun, ;2013;Cell reports;3;960-7; 23453967
Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration.;Hippenmeyer Simon, Youn Yong Ha, Moon Hyang Mi, Miyamichi Kazunari, Zong Hui, Wynshaw-Boris Anthony, Luo Liqun, ;2010;Neuron;68;695-709; 21092859

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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CD1;B6N-Igs5tm1(ACTB-tdTomato,-EGFP)Luo/Biat