C57BL/6NCrl-Churc1tm1b(EUCOMM)Wtsi/WtsiPh

Status

Under development - register interest

EMMA IDEM:14534
International strain nameC57BL/6NCrl-Churc1tm1b(EUCOMM)Wtsi/WtsiPh
Alternative nameEPD0511_1_B03
Strain typeTargeted Mutant Strains
Allele/Transgene symbolChurc1tm1b(EUCOMM)Wtsi
Gene/Transgene symbolChurc1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0511_1_B03. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using an X-linked CMV-Cre driver (EM:05414; C57BL/6N-Hprttm1(CMV-cre)Wtsi/WtsiOulu) for this purpose. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal heart morphology / IMPC
  • enlarged heart / IMPC
  • abnormal spleen morphology / IMPC
  • abnormal thymus morphology / IMPC
  • enlarged thymus / IMPC
  • increased startle reflex / IMPC
  • abnormal eye morphology / IMPC
  • increased effector memory T-helper cell number / IMPC
IMPC phenotypes (gene matching)
  • increased startle reflex / IMPC
  • enlarged thymus / IMPC
  • abnormal thymus morphology / IMPC
  • abnormal eye morphology / IMPC
  • enlarged heart / IMPC
  • abnormal spleen morphology / IMPC
  • abnormal heart morphology / IMPC
  • increased effector memory T-helper cell number / IMPC

Information on how we integrate external resources can be found here

Register interest

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

    Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

    * In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

    More details on pricing and delivery times

    Practical information

    Example health report
    (Current health report will be provided later)

    Material Transfer Agreement (MTA)
    Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

    EMMA conditions
    Legally binding conditions for the transfer

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