C57BL/6N-Sh3pxd2a^{tm1b(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:14517
International strain name	C57BL/6N-Sh3pxd2a^{tm1b(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0656_1_B12
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Sh3pxd2a^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Sh3pxd2a
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0656_1_B12. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6N

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating alkaline phosphatase level / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased circulating alkaline phosphatase level / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

abnormal basioccipital bone morphology / MGI
abnormal vertebral body morphology / MGI
abnormal interatrial septum morphology / MGI
double outlet right ventricle / MGI
abnormal atrioventricular cushion morphology / MGI
short snout / MGI
abnormal liver morphology / MGI
liver hypoplasia / MGI
enlarged liver sinusoidal spaces / MGI
abnormal pineal gland morphology / MGI
abnormal forebrain morphology / MGI
fused dorsal root ganglion / MGI
small superior cervical ganglion / MGI
abnormal testis morphology / MGI
abnormal lymphatic vessel morphology / MGI
abnormal muscle morphology / MGI
abnormal kidney morphology / MGI
abnormal brain morphology / MGI
abnormal artery morphology / MGI
abnormal nasal cavity morphology / MGI
abnormal nasal septum morphology / MGI
abnormal vomeronasal organ morphology / MGI
abnormal larynx morphology / MGI
persistent truncus arteriosis / MGI
abnormal semilunar valve morphology / MGI
small thyroid gland / MGI
dilated bile duct / MGI
bifid ureter / MGI
urinary bladder hypoplasia / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
diaphragmatic hernia / MGI
transposition of great arteries / MGI
abnormal adenohypophysis morphology / MGI
fetal growth retardation / MGI
abnormal optic cup morphology / MGI
basisphenoid bone foramen / MGI
abnormal vertebral arch morphology / MGI
fusion of vertebral arches / MGI
absent stapedial artery / MGI
abnormal occipital bone morphology / MGI
abnormal inferior vena cava morphology / MGI
abnormal heart position or orientation / MGI
absent styloid process / MGI
enlarged fourth ventricle / MGI
enlarged third ventricle / MGI
abnormal cervical rib / MGI
thoracoschisis / MGI
absent sublingual gland / MGI
abnormal right lung morphology / MGI
abnormal liver vasculature morphology / MGI
abnormal hyoid bone body morphology / MGI
atrial septal defect / MGI
ostium primum atrial septal defect / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
double inlet heart left ventricle / MGI
abnormal hepatic vein morphology / MGI
bicuspid aortic valve / MGI
abnormal pectinate muscle morphology / MGI
bicuspid pulmonary valve / MGI
cerebral arteriovenous malformation / MGI
persistent right dorsal aorta / MGI
abnormal aortic valve cusp morphology / MGI
abnormal pulmonary valve cusp morphology / MGI
fusion of atlas and occipital bones / MGI
abnormal lung position or orientation / MGI
herniated liver / MGI
pelvic kidney / MGI
double ureter / MGI
double outlet right ventricle with atrioventricular septal defect / MGI
dual inferior vena cava / MGI
abnormal basilar artery morphology / MGI
abnormal oral cavity morphology / MGI
absent hypoglossal canal / MGI
absent hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
abnormal eye muscle topology / MGI
retropharyngeal edema / MGI
retropleural edema / MGI
subcutaneous edema / MGI
abnormal Mullerian duct topology / MGI
absent celiac artery / MGI
abnormal stapedial artery topology / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve topology / MGI
persistent right 6th pharyngeal arch artery / MGI
small prevertebral sympathetic ganglia / MGI
abnormal vascular plexus formation / MGI
abnormal digastric muscle connection / MGI
abnormal olfactory bulb position / MGI
fragmented Meckel's cartilage / MGI
abnormal thymus topology / MGI
fusion of axis and occipital bones / MGI
abnormally deep median sulcus of tongue / MGI
abnormal infrahyoid muscle connection / MGI
reduced sympathetic cervical ganglion size / MGI
absent connection between subcutaneous lymph vessels and lymph sac / MGI
blood in lymph vessels / MGI
abnormal left vena cava superior connection / MGI
symmetric azygos veins / MGI
inverse situs of great intrathoracic arteries / MGI
abnormal vitelline vein topology / MGI
abnormal vertebral artery origin / MGI
absent canalicular internal carotid artery segment / MGI
absent parasellar internal carotid artery / MGI
abnormal ovary tissue architecture / MGI
abnormal Wolffian duct connection / MGI
embryo cyst / MGI
heterochrony / MGI
short tongue / MGI

MGI phenotypes (gene matching)

abnormal basioccipital bone morphology / MGI
abnormal vertebral body morphology / MGI
abnormal interatrial septum morphology / MGI
double outlet right ventricle / MGI
abnormal atrioventricular cushion morphology / MGI
short snout / MGI
abnormal liver morphology / MGI
liver hypoplasia / MGI
enlarged liver sinusoidal spaces / MGI
abnormal pineal gland morphology / MGI
abnormal forebrain morphology / MGI
fused dorsal root ganglion / MGI
small superior cervical ganglion / MGI
abnormal testis morphology / MGI
abnormal lymphatic vessel morphology / MGI
premature death / MGI
abnormal muscle morphology / MGI
abnormal kidney morphology / MGI
abnormal brain morphology / MGI
abnormal artery morphology / MGI
abnormal nasal cavity morphology / MGI
abnormal nasal septum morphology / MGI
abnormal vomeronasal organ morphology / MGI
abnormal larynx morphology / MGI
persistent truncus arteriosis / MGI
abnormal semilunar valve morphology / MGI
small thyroid gland / MGI
dilated bile duct / MGI
bifid ureter / MGI
urinary bladder hypoplasia / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
diaphragmatic hernia / MGI
transposition of great arteries / MGI
abnormal adenohypophysis morphology / MGI
fetal growth retardation / MGI
abnormal optic cup morphology / MGI
basisphenoid bone foramen / MGI
abnormal vertebral arch morphology / MGI
fusion of vertebral arches / MGI
absent stapedial artery / MGI
split xiphoid process / MGI
abnormal occipital bone morphology / MGI
abnormal inferior vena cava morphology / MGI
abnormal heart position or orientation / MGI
absent styloid process / MGI
enlarged fourth ventricle / MGI
enlarged third ventricle / MGI
abnormal cervical rib / MGI
thoracoschisis / MGI
absent sublingual gland / MGI
absent gastric milk in neonates / MGI
abnormal right lung morphology / MGI
abnormal liver vasculature morphology / MGI
cleft secondary palate / MGI
abnormal hyoid bone body morphology / MGI
atrial septal defect / MGI
ostium primum atrial septal defect / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
double inlet heart left ventricle / MGI
abnormal hepatic vein morphology / MGI
bicuspid aortic valve / MGI
abnormal pectinate muscle morphology / MGI
bicuspid pulmonary valve / MGI
cerebral arteriovenous malformation / MGI
persistent right dorsal aorta / MGI
abnormal aortic valve cusp morphology / MGI
abnormal pulmonary valve cusp morphology / MGI
fusion of atlas and occipital bones / MGI
mortality/aging / MGI
abnormal lung position or orientation / MGI
herniated liver / MGI
neonatal lethality, incomplete penetrance / MGI
perinatal lethality, incomplete penetrance / MGI
preweaning lethality, incomplete penetrance / MGI
pelvic kidney / MGI
double ureter / MGI
double outlet right ventricle with atrioventricular septal defect / MGI
dual inferior vena cava / MGI
abnormal basilar artery morphology / MGI
abnormal oral cavity morphology / MGI
absent hypoglossal canal / MGI
absent hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
abnormal eye muscle topology / MGI
retropharyngeal edema / MGI
retropleural edema / MGI
subcutaneous edema / MGI
abnormal Mullerian duct topology / MGI
absent celiac artery / MGI
abnormal stapedial artery topology / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve topology / MGI
persistent right 6th pharyngeal arch artery / MGI
small prevertebral sympathetic ganglia / MGI
abnormal vascular plexus formation / MGI
abnormal digastric muscle connection / MGI
abnormal olfactory bulb position / MGI
fragmented Meckel's cartilage / MGI
abnormal thymus topology / MGI
fusion of axis and occipital bones / MGI
abnormally deep median sulcus of tongue / MGI
abnormal infrahyoid muscle connection / MGI
reduced sympathetic cervical ganglion size / MGI
absent connection between subcutaneous lymph vessels and lymph sac / MGI
blood in lymph vessels / MGI
abnormal left vena cava superior connection / MGI
symmetric azygos veins / MGI
inverse situs of great intrathoracic arteries / MGI
abnormal vitelline vein topology / MGI
abnormal vertebral artery origin / MGI
absent canalicular internal carotid artery segment / MGI
absent parasellar internal carotid artery / MGI
abnormal ovary tissue architecture / MGI
abnormal Wolffian duct connection / MGI
embryo cyst / MGI
heterochrony / MGI
short tongue / MGI
persistent trigeminal artery / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Sh3pxd2atm1b(EUCOMM)Wtsi/WtsiCnbc