C57BL/6N-Kmt2dtm1.2(IMPC)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:14428
Citation informationRRID:IMSR_EM:14428 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Kmt2dtm1.2(IMPC)Wtsi/WtsiCnbc
Alternative nameBEPD0099_C03
Strain type
Allele/Transgene symbolKmt2dtm1.2(IMPC)Wtsi
Gene/Transgene symbolKmt2d
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from undef ES clone BEPD0099_C03. For further details on the construction of this clone see the page at the IMPC portal. ES cells were generated using the CRISPR/Cas9 technology. The details of the applied experimental procedures involving CRISPR/Cas9 technology are not available.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6N males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased leukocyte cell number / IMPC
  • decreased body length / IMPC
  • decreased erythrocyte cell number / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased circulating chloride level / IMPC
  • increased mean corpuscular hemoglobin / IMPC
  • decreased circulating sodium level / IMPC
  • increased circulating amylase level / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating chloride level / IMPC
  • decreased body length / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased erythrocyte cell number / IMPC
  • increased circulating amylase level / IMPC
  • decreased circulating sodium level / IMPC
  • decreased leukocyte cell number / IMPC
  • increased mean corpuscular hemoglobin / IMPC
MGI phenotypes (gene matching)
  • short maxilla / MGI
  • flattened snout / MGI
  • abnormal dentate gyrus morphology / MGI
  • decreased body weight / MGI
  • abnormal object recognition memory / MGI
  • abnormal inner ear canal morphology / MGI
  • abnormal hippocampus granule cell layer / MGI
  • impaired contextual conditioning behavior / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • impaired spatial learning / MGI

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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