C57BL/6N-Kmt2etm1.2(IMPC)Wtsi/WtsiOrl

Status

Available to order

EMMA IDEM:14411
International strain nameC57BL/6N-Kmt2etm1.2(IMPC)Wtsi/WtsiOrl
Alternative nameBEPD0111_B03
Strain type
Allele/Transgene symbolKmt2etm1.2(IMPC)Wtsi
Gene/Transgene symbolKmt2e
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from undef ES clone BEPD0111_B03. For further details on the construction of this clone see the page at the IMPC portal. ES cells were generated using the CRISPR/Cas9 technology. The details of the applied experimental procedures involving CRISPR/Cas9 technology are not available.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • increased circulating insulin level / IMPC
  • decreased lean body mass / IMPC
  • increased mean corpuscular hemoglobin / IMPC
  • increased blood urea nitrogen level / IMPC
  • increased mean corpuscular hemoglobin concentration / IMPC
  • absent pinna reflex / IMPC
  • increased total body fat amount / IMPC
  • increased red blood cell distribution width / IMPC
IMPC phenotypes (gene matching)
  • decreased lean body mass / IMPC
  • increased total body fat amount / IMPC
  • absent pinna reflex / IMPC
  • increased circulating insulin level / IMPC
  • increased mean corpuscular hemoglobin / IMPC
  • increased red blood cell distribution width / IMPC
  • increased blood urea nitrogen level / IMPC
  • increased mean corpuscular hemoglobin concentration / IMPC
MGI phenotypes (gene matching)
  • decreased hematocrit / MGI
  • increased neutrophil cell number / MGI
  • decreased leukocyte cell number / MGI
  • small spleen / MGI
  • spleen hypoplasia / MGI
  • small thymus / MGI
  • decreased thymocyte number / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • increased mortality induced by gamma-irradiation / MGI
  • postnatal growth retardation / MGI
  • thymus hypoplasia / MGI
  • reduced female fertility / MGI
  • male infertility / MGI
  • abnormal definitive hematopoiesis / MGI
  • small lymph nodes / MGI
  • abnormal bone marrow cell morphology/development / MGI
  • abnormal lymphopoiesis / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal myeloblast morphology/development / MGI
  • abnormal neutrophil differentiation / MGI
  • abnormal neutrophil physiology / MGI
  • low mean erythrocyte cell number / MGI
  • globozoospermia / MGI
  • decreased hemoglobin content / MGI
  • decreased erythrocyte cell number / MGI
  • no phenotypic analysis / MGI
  • thrombocytopenia / MGI
  • decreased macrophage cell number / MGI
  • abnormal hematopoietic stem cell morphology / MGI
  • increased hematopoietic stem cell number / MGI
  • decreased hematopoietic stem cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • decreased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • blepharitis / MGI
  • immune system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • decreased NK T cell number / MGI
  • decreased NK cell number / MGI
  • abnormal granulocyte differentiation / MGI
  • decreased pro-B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased common myeloid progenitor cell number / MGI
  • increased hemoglobin concentration distribution width / MGI
  • decreased total body fat amount / MGI
  • postnatal lethality, complete penetrance / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • lethality during fetal growth through weaning, incomplete penetrance / MGI
  • dilated seminal vesicles / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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