C57BL/6N-Bap1^{tm1c(EUCOMM)Hmgu}/WtsiPh

Status	Available to order
EMMA ID	EM:14399
International strain name	C57BL/6N-Bap1^{tm1c(EUCOMM)Hmgu}/WtsiPh
Alternative name	HEPD0526_2_G01
Strain type	Targeted Mutant Strains : Targeted Conditional
Allele/Transgene symbol	Bap1^{tm1c(EUCOMM)Hmgu}
Gene/Transgene symbol	Bap1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This line originates from EUCOMM ES clone HEPD0526_2_G01, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Uveal melanoma / Orphanet_39044
BAP1-related tumor predisposition syndrome / Orphanet_289539
Meningioma / Orphanet_2495
Pleural mesothelioma / Orphanet_50251
Non-specific syndromic intellectual disability / Orphanet_528084

IMPC phenotypes (gene matching)

increased basophil cell number / IMPC
abnormal auditory brainstem response / IMPC
preweaning lethality, complete penetrance / IMPC
abnormal bone structure / IMPC
increased eosinophil cell number / IMPC
abnormal vibrissa morphology / IMPC
abnormal vocalization / IMPC

MGI phenotypes (gene matching)

increased mammary adenocarcinoma incidence / MGI
no phenotypic analysis / MGI
embryonic growth retardation / MGI
increased squamous cell carcinoma incidence / MGI
increased incidence of tumors by chemical induction / MGI
hematopoietic system phenotype / MGI
increased ovary tumor incidence / MGI
increased lung carcinoma incidence / MGI
abnormal DNA methylation / MGI
increased skin tumor incidence / MGI
increased mesothelioma incidence / MGI
increased pancreatic islet cell carcinoma incidence / MGI
embryonic lethality between implantation and placentation, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
abnormal epigenetic regulation of gene expression / MGI
increased granulosa cell tumor incidence / MGI
increased lymphoma incidence / MGI
increased colon tumor incidence / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Bap1tm1c(EUCOMM)Hmgu/WtsiPh