C57BL/6N-Atm1Brd Fbf1tm1a(EUCOMM)Wtsi/WtsiCnrm

Status

Available to order

EMMA IDEM:14358
Citation informationRRID:IMSR_EM:14358 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Atm1Brd Fbf1tm1a(EUCOMM)Wtsi/WtsiCnrm
Alternative nameEPD0456_2_F09
Strain typeTargeted Mutant Strains
Allele/Transgene symbolFbf1tm1a(EUCOMM)Wtsi
Gene/Transgene symbolFbf1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0456_2_F09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased circulating LDL cholesterol level / IMPC
  • decreased circulating HDL cholesterol level / IMPC
  • increased leukocyte cell number / IMPC
  • increased circulating insulin level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • abnormal bone structure / IMPC
  • decreased lean body mass / IMPC
  • decreased circulating cholesterol level / IMPC
  • abnormal humerus morphology / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased total body fat amount / IMPC
  • decreased bone mineral content / IMPC
  • increased fasting circulating glucose level / IMPC
IMPC phenotypes (gene matching)
  • abnormal bone structure / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased circulating insulin level / IMPC
  • increased leukocyte cell number / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased circulating HDL cholesterol level / IMPC
  • increased total body fat amount / IMPC
  • decreased lean body mass / IMPC
  • decreased circulating LDL cholesterol level / IMPC
  • abnormal humerus morphology / IMPC
  • decreased bone mineral content / IMPC
  • increased fasting circulating glucose level / IMPC
  • decreased circulating cholesterol level / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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