C57BL/6N-Smg9^{tm1b(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:14340
International strain name	C57BL/6N-Smg9^{tm1b(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0428_2_D07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Smg9^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Smg9
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0428_2_D07. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

abnormal embryo size / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
exencephaly / IMPC
preweaning lethality, complete penetrance / IMPC
abnormal behavior / IMPC
decreased thigmotaxis / IMPC
hyperactivity / IMPC
decreased circulating glucose level / IMPC

MGI phenotypes (allele matching)

lordosis / MGI
abnormal interatrial septum morphology / MGI
double outlet right ventricle / MGI
abnormal atrioventricular cushion morphology / MGI
absent kidney / MGI
polydactyly / MGI
enlarged liver sinusoidal spaces / MGI
abnormal pineal gland morphology / MGI
exencephaly / MGI
absent superior cervical ganglion / MGI
small superior cervical ganglion / MGI
anophthalmia / MGI
abnormal lens morphology / MGI
abnormal nasal cavity morphology / MGI
abnormal vein morphology / MGI
aphakia / MGI
liver cysts / MGI
aorta coarctation / MGI
abnormal eye muscle morphology / MGI
atrium hypoplasia / MGI
abnormal neurohypophysis morphology / MGI
abnormal optic cup morphology / MGI
basisphenoid bone foramen / MGI
fusion of vertebral bodies / MGI
abnormal middle ear ossicle morphology / MGI
coloboma / MGI
abnormal endolymphatic duct morphology / MGI
abnormal inferior vena cava morphology / MGI
abnormal heart position or orientation / MGI
abnormal cervical rib / MGI
thoracoschisis / MGI
abnormal optic chiasm morphology / MGI
absent optic chiasm / MGI
atrioventricular septal defect / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
abnormal inferior vena cava valve morphology / MGI
persistent right dorsal aorta / MGI
left atrium hypoplasia / MGI
double ureter / MGI
interrupted aortic arch, type b / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
subcutaneous edema / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve morphology / MGI
duplication of ductus venosus / MGI
persistent right 6th pharyngeal arch artery / MGI
reduced sympathetic cervical ganglion size / MGI
absent connection between subcutaneous lymph vessels and lymph sac / MGI
blood in lymph vessels / MGI
heterochrony / MGI

MGI phenotypes (gene matching)

lordosis / MGI
abnormal interatrial septum morphology / MGI
double outlet right ventricle / MGI
abnormal atrioventricular cushion morphology / MGI
absent kidney / MGI
polydactyly / MGI
enlarged liver sinusoidal spaces / MGI
abnormal pineal gland morphology / MGI
exencephaly / MGI
absent superior cervical ganglion / MGI
small superior cervical ganglion / MGI
anophthalmia / MGI
microphthalmia / MGI
abnormal lens morphology / MGI
edema / MGI
hemorrhage / MGI
abnormal nasal cavity morphology / MGI
thin myocardium / MGI
abnormal vein morphology / MGI
aphakia / MGI
liver cysts / MGI
aorta coarctation / MGI
abnormal eye muscle morphology / MGI
atrium hypoplasia / MGI
abnormal neurohypophysis morphology / MGI
decreased fetal size / MGI
fetal growth retardation / MGI
abnormal optic cup morphology / MGI
basisphenoid bone foramen / MGI
fusion of vertebral bodies / MGI
abnormal middle ear ossicle morphology / MGI
coloboma / MGI
abnormal endolymphatic duct morphology / MGI
abnormal inferior vena cava morphology / MGI
abnormal heart position or orientation / MGI
abnormal cervical rib / MGI
thoracoschisis / MGI
preaxial polydactyly / MGI
abnormal optic chiasm morphology / MGI
absent optic chiasm / MGI
atrioventricular septal defect / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
abnormal inferior vena cava valve morphology / MGI
persistent right dorsal aorta / MGI
left atrium hypoplasia / MGI
postnatal lethality, complete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
double ureter / MGI
interrupted aortic arch, type b / MGI
decreased midbrain size / MGI
decreased hindbrain size / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
subcutaneous edema / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve morphology / MGI
duplication of ductus venosus / MGI
persistent right 6th pharyngeal arch artery / MGI
reduced sympathetic cervical ganglion size / MGI
absent connection between subcutaneous lymph vessels and lymph sac / MGI
blood in lymph vessels / MGI
heterochrony / MGI
abnormal lens topology / MGI
persistent trigeminal artery / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

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C57BL/6N-Smg9tm1b(EUCOMM)Wtsi/WtsiH