C57BL/6N;C57BL/6NTac-Ssr2tm1b(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:14321
International strain nameC57BL/6N;C57BL/6NTac-Ssr2tm1b(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0654_2_C10
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSsr2tm1b(EUCOMM)Wtsi
Gene/Transgene symbolSsr2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0654_2_C10. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6N C57BL/6NTac

Disease and phenotype information

MGI phenotypes (allele matching)
  • scoliosis / MGI
  • abnormal interatrial septum morphology / MGI
  • double outlet right ventricle / MGI
  • enlarged liver sinusoidal spaces / MGI
  • abnormal thymus morphology / MGI
  • abnormal forebrain morphology / MGI
  • dilated lateral ventricles / MGI
  • small superior cervical ganglion / MGI
  • abnormal optic nerve morphology / MGI
  • intracerebral hemorrhage / MGI
  • abnormal diaphragm morphology / MGI
  • persistent truncus arteriosis / MGI
  • abnormal eye muscle morphology / MGI
  • abnormal Wolffian duct morphology / MGI
  • thin myocardium compact layer / MGI
  • fetal growth retardation / MGI
  • abnormal optic stalk morphology / MGI
  • abnormal optic cup morphology / MGI
  • basisphenoid bone foramen / MGI
  • fusion of vertebral arches / MGI
  • hemopericardium / MGI
  • enlarged third ventricle / MGI
  • abnormal optic chiasm morphology / MGI
  • abnormal hyoid bone greater horn morphology / MGI
  • perimembraneous ventricular septal defect / MGI
  • muscular ventricular septal defect / MGI
  • abnormal inferior vena cava valve morphology / MGI
  • common truncal valve / MGI
  • abnormal atrioventricular septum morphology / MGI
  • abnormal pulmonary valve cusp morphology / MGI
  • interrupted aortic arch, type b / MGI
  • abnormal digastric muscle morphology / MGI
  • abnormal hypoglossal nerve topology / MGI
  • subcutaneous edema / MGI
  • trigeminal neuroma / MGI
  • absent ductus venosus valve / MGI
  • abnormal ductus venosus valve morphology / MGI
  • abnormal infrahyoid muscle connection / MGI
  • reduced sympathetic cervical ganglion size / MGI
  • abnormal hepatic vein connection / MGI
  • heterochrony / MGI
MGI phenotypes (gene matching)
  • scoliosis / MGI
  • abnormal interatrial septum morphology / MGI
  • double outlet right ventricle / MGI
  • enlarged liver sinusoidal spaces / MGI
  • abnormal thymus morphology / MGI
  • abnormal forebrain morphology / MGI
  • dilated lateral ventricles / MGI
  • small superior cervical ganglion / MGI
  • abnormal optic nerve morphology / MGI
  • intracerebral hemorrhage / MGI
  • abnormal diaphragm morphology / MGI
  • persistent truncus arteriosis / MGI
  • abnormal eye muscle morphology / MGI
  • abnormal Wolffian duct morphology / MGI
  • thin myocardium compact layer / MGI
  • fetal growth retardation / MGI
  • abnormal optic stalk morphology / MGI
  • abnormal optic cup morphology / MGI
  • basisphenoid bone foramen / MGI
  • fusion of vertebral arches / MGI
  • hemopericardium / MGI
  • enlarged third ventricle / MGI
  • abnormal optic chiasm morphology / MGI
  • abnormal hyoid bone greater horn morphology / MGI
  • perimembraneous ventricular septal defect / MGI
  • muscular ventricular septal defect / MGI
  • abnormal inferior vena cava valve morphology / MGI
  • common truncal valve / MGI
  • abnormal atrioventricular septum morphology / MGI
  • abnormal pulmonary valve cusp morphology / MGI
  • interrupted aortic arch, type b / MGI
  • abnormal digastric muscle morphology / MGI
  • abnormal hypoglossal nerve topology / MGI
  • subcutaneous edema / MGI
  • trigeminal neuroma / MGI
  • absent ductus venosus valve / MGI
  • abnormal ductus venosus valve morphology / MGI
  • abnormal infrahyoid muscle connection / MGI
  • reduced sympathetic cervical ganglion size / MGI
  • abnormal hepatic vein connection / MGI
  • heterochrony / MGI

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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