C57BL/6N-Smg1^{tm1b(EUCOMM)Hmgu}/WtsiH

Status	Available to order
EMMA ID	EM:14316
International strain name	C57BL/6N-Smg1^{tm1b(EUCOMM)Hmgu}/WtsiH
Alternative name	HEPD0860_6_F10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Smg1^{tm1b(EUCOMM)Hmgu}
Gene/Transgene symbol	Smg1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0860_6_F10. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

IMPC phenotypes (allele matching)

IMPC phenotypes (gene matching)

MGI phenotypes (gene matching)

abnormal tooth development / MGI
abnormal heart development / MGI
decreased cell proliferation / MGI
enlarged liver / MGI
enlarged spleen / MGI
increased body length / MGI
increased body weight / MGI
increased body size / MGI
abnormal eye development / MGI
internal hemorrhage / MGI
decreased embryo size / MGI
embryonic growth arrest / MGI
delayed brain development / MGI
increased tumor incidence / MGI
increased B cell derived lymphoma incidence / MGI
increased lung adenocarcinoma incidence / MGI
premature death / MGI
abnormal tooth morphology / MGI
abnormal kidney morphology / MGI
chronic inflammation / MGI
hepatic steatosis / MGI
abnormal cardiovascular development / MGI
delayed embryo turning / MGI
failure of heart looping / MGI
homeostasis/metabolism phenotype / MGI
cellular phenotype / MGI
skeleton phenotype / MGI
hematopoietic system phenotype / MGI
abnormal cell physiology / MGI
increased circulating interleukin-6 level / MGI
decreased circulating interleukin-1 beta level / MGI
abnormal basicranium angle / MGI
prognathia / MGI
embryonic lethality between implantation and placentation, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
increased lymphoma incidence / MGI
increased embryonic tissue cell apoptosis / MGI
absent optic pit / MGI

Information from provider