C57BL/6N-Aicda^{tm1b(EUCOMM)Hmgu}/WtsiCnbc

Status	Available to order
EMMA ID	EM:14269
International strain name	C57BL/6N-Aicda^{tm1b(EUCOMM)Hmgu}/WtsiCnbc
Alternative name	HEPD0615_4_B08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Aicda^{tm1b(EUCOMM)Hmgu}
Gene/Transgene symbol	Aicda
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0615_4_B08. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6N

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)

IMPC phenotypes (gene matching)

MGI phenotypes (gene matching)

abnormal humoral immune response / MGI
decreased IgG level / MGI
decreased IgA level / MGI
no abnormal phenotype detected / MGI
increased susceptibility to bacterial infection / MGI
decreased IgE level / MGI
increased IgM level / MGI
no phenotypic analysis / MGI
abnormal somatic hypermutation frequency / MGI
abnormal class switch recombination / MGI
immune system phenotype / MGI
increased germinal center B cell number / MGI
decreased IgG1 level / MGI
decreased IgG2a level / MGI
decreased IgG2b level / MGI
decreased IgG3 level / MGI
chromosomal instability / MGI
increased susceptibility to bacterial infection induced morbidity/mortality / MGI
abnormal gut flora balance / MGI

Information from provider