C57BL/6N-Myh3^{tm1b(EUCOMM)Wtsi}/WtsiH

Status	Only small colony available
EMMA ID	EM:14254
International strain name	C57BL/6N-Myh3^{tm1b(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0780_2_F11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Myh3^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Myh3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0780_2_F11. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using an X-linked CMV-Cre driver (EM:05414; C57BL/6N-Hprt^{tm1(CMV-cre)Wtsi}/WtsiOulu) for this purpose. Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Sheldon-Hall syndrome / Orphanet_1147
Digitotalar dysmorphism / Orphanet_1146
Freeman-Sheldon syndrome / Orphanet_2053
Spondylocarpotarsal synostosis / Orphanet_3275
Autosomal recessive multiple pterygium syndrome / Orphanet_2990
Autosomal dominant multiple pterygium syndrome / Orphanet_65743

Information on how we integrate external resources can be found here

Order (limited)

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

C57BL/6N-Myh3tm1b(EUCOMM)Wtsi/WtsiH