C57BL/6N-H13^{tm1b(KOMP)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:14250
International strain name	C57BL/6N-H13^{tm1b(KOMP)Wtsi}/WtsiH
Alternative name	EPD0867_5_D04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	H13^{tm1b(KOMP)Wtsi}
Gene/Transgene symbol	H13
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0867_5_D04. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on KOMP final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal snout morphology / IMPC
increased bone mineral content / IMPC
preweaning lethality, complete penetrance / IMPC
decreased fasting circulating glucose level / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC
decreased fasting circulating glucose level / IMPC
increased bone mineral content / IMPC
abnormal snout morphology / IMPC

MGI phenotypes (allele matching)

abnormal vertebrae morphology / MGI
abnormal rib morphology / MGI
absent ribs / MGI
abnormal interatrial septum morphology / MGI
double outlet right ventricle / MGI
absent kidney / MGI
enlarged liver sinusoidal spaces / MGI
abnormal forebrain morphology / MGI
abnormal olfactory bulb morphology / MGI
abnormal hindbrain morphology / MGI
exencephaly / MGI
fused dorsal root ganglion / MGI
absent superior cervical ganglion / MGI
small superior cervical ganglion / MGI
abnormal tail morphology / MGI
abnormal brain morphology / MGI
abnormal outer ear morphology / MGI
situs inversus / MGI
abnormal posterior semicircular canal morphology / MGI
small thyroid gland / MGI
small kidney / MGI
left pulmonary isomerism / MGI
abnormal forebrain development / MGI
abnormal nervous system morphology / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal Wolffian duct morphology / MGI
transposition of great arteries / MGI
retroesophageal right subclavian artery / MGI
abnormal optic stalk morphology / MGI
abnormal optic cup morphology / MGI
abnormal vertebral arch morphology / MGI
abnormal cervical atlas morphology / MGI
abnormal stapedial artery morphology / MGI
absent stapedial artery / MGI
decreased length of long bones / MGI
absent Mullerian ducts / MGI
abnormal styloid process morphology / MGI
abnormal brain internal capsule morphology / MGI
thoracoschisis / MGI
abnormal optic chiasm morphology / MGI
abnormal liver vasculature morphology / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
abnormal hepatic vein morphology / MGI
abnormal inferior vena cava valve morphology / MGI
abnormal pulmonary valve cusp morphology / MGI
abnormal hypoglossal canal morphology / MGI
absent hypoglossal canal / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
subcutaneous edema / MGI
absent celiac artery / MGI
abnormal azygos vein topology / MGI
abnormal vitelline vein connection / MGI
abnormal pancreas topology / MGI
enlarged paraumbilical vein / MGI
abnormal stapedial artery topology / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve morphology / MGI
abnormal brachial plexus formation / MGI
abnormal thymus topology / MGI
abnormal infrahyoid muscle morphology / MGI
abnormal infrahyoid muscle connection / MGI
multiple persisting craniopharyngeal ducts / MGI
reduced sympathetic cervical ganglion size / MGI
blood in lymph vessels / MGI
symmetric azygos veins / MGI
abnormal umbilical vein topology / MGI
abnormal vitelline vein topology / MGI
persistent dorsal ophthalmic artery / MGI
abnormal vertebral artery origin / MGI
absent posterior communicating artery / MGI
embryo tumor / MGI
heterochrony / MGI
abnormal intestine placement / MGI

MGI phenotypes (gene matching)

abnormal vertebrae morphology / MGI
abnormal rib morphology / MGI
absent ribs / MGI
abnormal interatrial septum morphology / MGI
double outlet right ventricle / MGI
absent kidney / MGI
enlarged liver sinusoidal spaces / MGI
abnormal forebrain morphology / MGI
abnormal olfactory bulb morphology / MGI
abnormal hindbrain morphology / MGI
exencephaly / MGI
fused dorsal root ganglion / MGI
absent superior cervical ganglion / MGI
small superior cervical ganglion / MGI
abnormal tail morphology / MGI
abnormal brain morphology / MGI
abnormal outer ear morphology / MGI
situs inversus / MGI
abnormal posterior semicircular canal morphology / MGI
small thyroid gland / MGI
small kidney / MGI
left pulmonary isomerism / MGI
abnormal forebrain development / MGI
abnormal nervous system morphology / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal Wolffian duct morphology / MGI
transposition of great arteries / MGI
retroesophageal right subclavian artery / MGI
abnormal optic stalk morphology / MGI
abnormal optic cup morphology / MGI
abnormal vertebral arch morphology / MGI
abnormal cervical atlas morphology / MGI
abnormal stapedial artery morphology / MGI
absent stapedial artery / MGI
decreased length of long bones / MGI
absent Mullerian ducts / MGI
abnormal styloid process morphology / MGI
abnormal brain internal capsule morphology / MGI
thoracoschisis / MGI
abnormal optic chiasm morphology / MGI
abnormal liver vasculature morphology / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
abnormal hepatic vein morphology / MGI
abnormal inferior vena cava valve morphology / MGI
abnormal pulmonary valve cusp morphology / MGI
abnormal hypoglossal canal morphology / MGI
absent hypoglossal canal / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
subcutaneous edema / MGI
absent celiac artery / MGI
abnormal azygos vein topology / MGI
abnormal vitelline vein connection / MGI
abnormal pancreas topology / MGI
enlarged paraumbilical vein / MGI
abnormal stapedial artery topology / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve morphology / MGI
abnormal brachial plexus formation / MGI
abnormal thymus topology / MGI
abnormal infrahyoid muscle morphology / MGI
abnormal infrahyoid muscle connection / MGI
multiple persisting craniopharyngeal ducts / MGI
reduced sympathetic cervical ganglion size / MGI
blood in lymph vessels / MGI
symmetric azygos veins / MGI
abnormal umbilical vein topology / MGI
abnormal vitelline vein topology / MGI
persistent dorsal ophthalmic artery / MGI
abnormal vertebral artery origin / MGI
absent posterior communicating artery / MGI
embryo tumor / MGI
heterochrony / MGI
abnormal intestine placement / MGI
absent inferior vena cava / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

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C57BL/6N-H13tm1b(KOMP)Wtsi/WtsiH