C57BL/6N-Fam163atm2b(KOMP)Wtsi/WtsiOrl

Status

Available to order

EMMA IDEM:14249
International strain nameC57BL/6N-Fam163atm2b(KOMP)Wtsi/WtsiOrl
Alternative nameEPD0830_6_B05
Strain typeTargeted Mutant Strains
Allele/Transgene symbolFam163atm2b(KOMP)Wtsi
Gene/Transgene symbolFam163a
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0830_6_B05. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on KOMP final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased leukocyte cell number / IMPC
  • decreased body length / IMPC
  • abnormal iris morphology / IMPC
  • mydriasis / IMPC
  • increased hematocrit / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased erythrocyte cell number / IMPC
  • increased lean body mass / IMPC
  • improved glucose tolerance / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • increased hemoglobin content / IMPC
  • increased circulating total protein level / IMPC
  • decreased total body fat amount / IMPC
IMPC phenotypes (gene matching)
  • increased erythrocyte cell number / IMPC
  • decreased body length / IMPC
  • increased hematocrit / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • increased leukocyte cell number / IMPC
  • increased lean body mass / IMPC
  • mydriasis / IMPC
  • increased hemoglobin content / IMPC
  • improved glucose tolerance / IMPC
  • increased circulating total protein level / IMPC
  • decreased total body fat amount / IMPC
  • abnormal iris morphology / IMPC
  • increased circulating alkaline phosphatase level / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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