C57BL/6N-Kif3btm1b(EUCOMM)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:14245
International strain nameC57BL/6N-Kif3btm1b(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0619_3_A05
Strain typeTargeted Mutant Strains
Allele/Transgene symbolKif3btm1b(EUCOMM)Wtsi
Gene/Transgene symbolKif3b
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0619_3_A05. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using an X-linked CMV-Cre driver (EM:05414; C57BL/6N-Hprttm1(CMV-cre)Wtsi/WtsiOulu) for this purpose. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased grip strength / IMPC
  • increased bone mineral content / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • prenatal lethality prior to heart atrial septation / IMPC
IMPC phenotypes (gene matching)
  • prenatal lethality prior to heart atrial septation / IMPC
  • increased bone mineral content / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • increased grip strength / IMPC
MGI phenotypes (gene matching)
  • distended pericardium / MGI
  • exencephaly / MGI
  • open neural tube / MGI
  • abnormal spinal cord morphology / MGI
  • decreased embryo size / MGI
  • incomplete embryo turning / MGI
  • abnormal left-right axis patterning / MGI
  • absent embryonic cilia / MGI
  • abnormal direction of heart looping / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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