C57BL/6N-Ncf2^{tm1c(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:14211
International strain name	C57BL/6N-Ncf2^{tm1c(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0240_5_B03
Strain type	Targeted Mutant Strains : Targeted Conditional
Allele/Transgene symbol	Ncf2^{tm1c(EUCOMM)Wtsi}
Gene/Transgene symbol	Ncf2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This line originates from EUCOMM ES clone EPD0240_5_B03, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)

MGI phenotypes (gene matching)

increased activated T cell number / MGI
lung inflammation / MGI
premature death / MGI
abnormal spleen germinal center morphology / MGI
increased urine protein level / MGI
abnormal redox activity / MGI
increased anti-double stranded DNA antibody level / MGI
increased susceptibility to systemic lupus erythematosus / MGI
increased B cell number / MGI
increased T cell number / MGI
abnormal renal glomerulus morphology / MGI
increased susceptibility to fungal infection / MGI
increased CD8-positive, alpha-beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
increased follicular B cell number / MGI
increased germinal center B cell number / MGI
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / MGI
increased CD4-positive, alpha-beta memory T cell number / MGI
abnormal mesangial matrix morphology / MGI
abnormal kidney interstitium morphology / MGI
glomerular crescent / MGI
renal glomerular protein deposits / MGI
renal glomerular immunoglobulin deposits / MGI

Information from provider