B6Brd;B6Dnk;B6N-Tyrc-Brd-Crlf3tm1c(KOMP)Wtsi/WtsiPh

Status

Under development - register interest

EMMA IDEM:14203
International strain nameB6Brd;B6Dnk;B6N-Tyrc-Brd-Crlf3tm1c(KOMP)Wtsi/WtsiPh
Alternative nameEPD0033_3_C11
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolCrlf3tm1c(KOMP)Wtsi
Gene/Transgene symbolCrlf3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis line originates from KOMP ES clone EPD0033_3_C11, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased circulating iron level / IMPC
  • decreased body weight / IMPC
  • thrombocytopenia / IMPC
  • increased red blood cell distribution width / IMPC
  • abnormal behavior / IMPC
  • decreased mean corpuscular volume / IMPC
  • tremors / IMPC
MGI phenotypes (gene matching)
  • decreased body weight / MGI
  • decreased lean body mass / MGI
  • increased hemoglobin content / MGI
  • increased mean corpuscular hemoglobin concentration / MGI
  • decreased mean corpuscular hemoglobin concentration / MGI

Information on how we integrate external resources can be found here

Register interest

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

    Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

    * In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

    More details on pricing and delivery times

    Practical information

    Genotyping protocol

    Example health report
    (Current health report will be provided later)

    Material Transfer Agreement (MTA)
    Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

    EMMA conditions
    Legally binding conditions for the transfer

    Other EMMA strains

    Not found what you were looking for? Search here for other strains available from EMMA.


    Search
    INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).