C57BL/6N-Kmt2b^{tm1.2(IMPC)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:14200
International strain name	C57BL/6N-Kmt2b^{tm1.2(IMPC)Wtsi}/WtsiH
Alternative name	BEPD0107_E07
Strain type
Allele/Transgene symbol	Kmt2b^{tm1.2(IMPC)Wtsi}
Gene/Transgene symbol	Kmt2b
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from undef ES clone BEPD0107_E07. For further details on the construction of this clone see the page at the IMPC portal. ES cells were generated using the CRISPR/Cas9 technology. The details of the applied experimental procedures involving CRISPR/Cas9 technology are not available.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)

IMPC phenotypes (gene matching)

MGI phenotypes (gene matching)

abnormal heart looping / MGI
abnormal head morphology / MGI
abnormal hepatocyte morphology / MGI
exencephaly / MGI
open neural tube / MGI
increased circulating triglyceride level / MGI
abnormal embryo development / MGI
abnormal somite development / MGI
abnormal embryo turning / MGI
embryonic growth arrest / MGI
edema / MGI
reduced female fertility / MGI
increased circulating insulin level / MGI
no abnormal phenotype detected / MGI
hepatic steatosis / MGI
increased liver weight / MGI
decreased insulin secretion / MGI
kinked neural tube / MGI
embryonic growth retardation / MGI
increased circulating cholesterol level / MGI
impaired glucose tolerance / MGI
pericardial effusion / MGI
insulin resistance / MGI
liver/biliary system phenotype / MGI
cellular phenotype / MGI
increased circulating glucose level / MGI
increased apoptosis / MGI
abnormal DNA methylation / MGI
enlarged pancreatic islets / MGI
increased epididymal fat pad weight / MGI
decreased epididymal fat pad weight / MGI
increased liver triglyceride level / MGI
increased total body fat amount / MGI
increased liver free fatty acids level / MGI
perinatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
embryonic lethality, incomplete penetrance / MGI
embryonic lethality between somite formation and embryo turning, incomplete penetrance / MGI
lethality during fetal growth through weaning, incomplete penetrance / MGI
rostral body truncation / MGI

Information from provider