C57BL/6NTac-Vangl1^{tm1e(KOMP)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:14047
International strain name	C57BL/6NTac-Vangl1^{tm1e(KOMP)Wtsi}/WtsiIeg
Alternative name	EPD0164_3_G07
Strain type	Targeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbol	Vangl1^{tm1e(KOMP)Wtsi}
Gene/Transgene symbol	Vangl1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0164_3_G07. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on KOMP final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.;DiTommaso Tia, Jones Lynelle K, Cottle Denny L, null null, Gerdin Anna-Karin, Vancollie Valerie E, Watt Fiona M, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, Sundberg John P, White Jacqueline K, Smyth Ian M, ;2014;PLoS genetics;10;e1004705; 25340873

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating aspartate transaminase level / IMPC
thrombocytosis / IMPC
increased mean corpuscular hemoglobin / IMPC
preweaning lethality, incomplete penetrance / IMPC
hyperplasia / IMPC
process of degenerative change / IMPC
extramedullary hemopoiesis / IMPC

IMPC phenotypes (gene matching)

extramedullary hemopoiesis / IMPC
hyperplasia / IMPC
process of degenerative change / IMPC
preweaning lethality, incomplete penetrance / IMPC
thrombocytosis / IMPC
increased mean corpuscular hemoglobin / IMPC
increased circulating aspartate transaminase level / IMPC

MGI phenotypes (allele matching)

increased mean corpuscular hemoglobin / MGI
increased bone mineral content / MGI

MGI phenotypes (gene matching)

no abnormal phenotype detected / MGI
nervous system phenotype / MGI
abnormal orientation of cochlear hair cell stereociliary bundles / MGI
cardiovascular system phenotype / MGI
increased mean corpuscular hemoglobin / MGI
increased bone mineral content / MGI

Literature references

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.;DiTommaso Tia, Jones Lynelle K, Cottle Denny L, null null, Gerdin Anna-Karin, Vancollie Valerie E, Watt Fiona M, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, Sundberg John P, White Jacqueline K, Smyth Ian M, ;2014;PLoS genetics;10;e1004705; 25340873

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Vangl1tm1e(KOMP)Wtsi/WtsiIeg