C57BL/6N-Atm1Brd Ltbtm2(EGFP/cre/ERT2)Wtsi/WtsiIeg

Status

Available to order

EMMA IDEM:13991
International strain nameC57BL/6N-Atm1Brd Ltbtm2(EGFP/cre/ERT2)Wtsi/WtsiIeg
Alternative nameCEPD0096_5_B05
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolLtbtm2(EGFP/cre/ERT2)Wtsi
Gene/Transgene symbolLtb
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone CEPD0096_5_B05. For further details on the construction of this clone see the page at the IMPC portal.
Quality Control/Disclaimer: The structure of the targeted mutation is not fully verified. It is recommended that the recipient confirms the mutation structure by Southern blotting. The Cre expression pattern is expected to mirror that of the host gene, but this may not be fully described and/or may be modified in the targeted allele. Although characterisation of the Cre expression pattern is an objective of EUCOMMTools this is an ongoing process which may be incomplete. It is therefore recommended that the recipient verifies the Cre expression pattern. For the tm2(EGFP/Cre/ERT2) type allele, if the promoter-driven selection cassette is flanked with Rox sites, it may be removed using Dre recombinase. Please be aware that the strain description may be subject to change. For the most recent description please consult the information pages for the appropriate product details at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

MGI phenotypes (gene matching)
  • increased leukocyte cell number / MGI
  • abnormal colon morphology / MGI
  • abnormal liver morphology / MGI
  • abnormal spleen morphology / MGI
  • spleen hyperplasia / MGI
  • abnormal lung morphology / MGI
  • weight loss / MGI
  • abnormal humoral immune response / MGI
  • decreased IgG level / MGI
  • decreased IgA level / MGI
  • abnormal spleen red pulp morphology / MGI
  • abnormal spleen white pulp morphology / MGI
  • abnormal spleen germinal center morphology / MGI
  • abnormal spleen marginal zone morphology / MGI
  • increased susceptibility to bacterial infection / MGI
  • increased IgM level / MGI
  • colitis / MGI
  • absent Peyer's patches / MGI
  • no phenotypic analysis / MGI
  • abnormal class switch recombination / MGI
  • increased lymphocyte cell number / MGI
  • increased susceptibility to parasitic infection / MGI
  • abnormal mesenteric lymph node morphology / MGI
  • absent lymph nodes / MGI
  • absent germinal center B cells / MGI
  • absent follicular dendritic cells / MGI
  • absent spleen marginal zone / MGI
  • absent peripheral lymph nodes / MGI
  • absent mesenteric lymph nodes / MGI
  • abnormal spleen B cell follicle morphology / MGI
  • abnormal spleen follicular dendritic cell network / MGI
  • absent spleen germinal center / MGI
  • intermingled spleen red and white pulp / MGI
  • decreased spleen white pulp amount / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • absent inguinal lymph nodes / MGI
  • absent brachial lymph nodes / MGI
  • absent axillary lymph nodes / MGI
  • absent cervical lymph nodes / MGI
  • absent popliteal lymph nodes / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • increased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • increased susceptibility to bacterial infection induced morbidity/mortality / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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