C57BL/6NTac-Prkab1^{tm1a(KOMP)Wtsi}/WtsiPh

Status	Available to order
EMMA ID	EM:13978
International strain name	C57BL/6NTac-Prkab1^{tm1a(KOMP)Wtsi}/WtsiPh
Alternative name	EPD0033_3_C09
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Prkab1^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Prkab1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0033_3_C09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131 Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.;DiTommaso Tia, Jones Lynelle K, Cottle Denny L, null null, Gerdin Anna-Karin, Vancollie Valerie E, Watt Fiona M, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, Sundberg John P, White Jacqueline K, Smyth Ian M, ;2014;PLoS genetics;10;e1004705; 25340873

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased hematocrit / IMPC
abnormal response to new environment / IMPC
abnormal retina vasculature morphology / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
increased circulating alkaline phosphatase level / IMPC
increased circulating bilirubin level / IMPC
abnormal retina blood vessel pattern / IMPC

IMPC phenotypes (gene matching)

decreased fasting circulating glucose level / IMPC
decreased hematocrit / IMPC
increased memory-marker gamma-delta T cell number / IMPC
enlarged spleen / IMPC
decreased grip strength / IMPC
increased glycosylated hemoglobin level / IMPC
decreased mean corpuscular volume / IMPC
decreased hemoglobin content / IMPC
decreased total retina thickness / IMPC
hyperplasia / IMPC
increased spleen weight / IMPC
absent pinna reflex / IMPC
enlarged heart / IMPC
abnormal spleen morphology / IMPC
impaired glucose tolerance / IMPC
enlarged testis / IMPC
increased memory-marker CD4-negative NK T cell number / IMPC
abnormal epididymis morphology / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
decreased circulating cholesterol level / IMPC
increased red blood cell distribution width / IMPC
abnormal thymus morphology / IMPC
decreased Ly6C-positive mature NK cell number / IMPC
abnormal response to new environment / IMPC
increased fasting circulating glucose level / IMPC
decreased circulating HDL cholesterol level / IMPC
increased heart weight / IMPC
decreased circulating phosphate level / IMPC
decreased erythrocyte cell number / IMPC
abnormal retina blood vessel morphology / IMPC
decreased circulating amylase level / IMPC
preweaning lethality, incomplete penetrance / IMPC
fibro-osseous lesion / IMPC
decreased mean corpuscular hemoglobin / IMPC
shortened RR interval / IMPC
enlarged epididymis / IMPC
increased circulating bilirubin level / IMPC
cataract / IMPC
increased neutrophil cell number / IMPC
abnormal heart left ventricle morphology / IMPC
abnormal heart morphology / IMPC
abnormal freezing behavior / IMPC
abnormal retina inner nuclear layer morphology / IMPC
decreased circulating LDL cholesterol level / IMPC
abnormal lens morphology / IMPC
enlarged kidney / IMPC
increased circulating alkaline phosphatase level / IMPC
impaired contextual conditioning behavior / IMPC
decreased circulating iron level / IMPC
abnormal kidney morphology / IMPC
no spontaneous movement / IMPC
decreased lymphocyte cell number / IMPC
abnormal retina vasculature morphology / IMPC
increased mean platelet volume / IMPC
enlarged thymus / IMPC
decreased body weight / IMPC
decreased circulating chloride level / IMPC
increased lung compliance / IMPC
abnormal retina blood vessel pattern / IMPC
decreased kidney weight / IMPC
decreased bone mineral density / IMPC
decreased lung elastance / IMPC
increased heart rate / IMPC
decreased bone mineral content / IMPC
abnormal testis morphology / IMPC
anophthalmia / IMPC
increased circulating alanine transaminase level / IMPC

MGI phenotypes (allele matching)

decreased hematocrit / MGI
decreased hemoglobin content / MGI
decreased erythrocyte cell number / MGI
increased circulating bilirubin level / MGI
decreased mean corpuscular hemoglobin / MGI
abnormal inferior vena cava morphology / MGI
decreased circulating magnesium level / MGI
abnormal ductus venosus morphology / MGI

MGI phenotypes (gene matching)

decreased hematocrit / MGI
extramedullary hematopoiesis / MGI
abnormal erythropoiesis / MGI
echinocytosis / MGI
schistocytosis / MGI
enlarged spleen / MGI
tremors / MGI
decreased brain size / MGI
abnormal cerebral cortex morphology / MGI
abnormal dentate gyrus morphology / MGI
abnormal cerebellum morphology / MGI
absent cerebellar granule layer / MGI
abnormal myelination / MGI
decreased body weight / MGI
abnormal optic nerve morphology / MGI
ataxia / MGI
abnormal lipid level / MGI
hemolytic anemia / MGI
seizures / MGI
no abnormal phenotype detected / MGI
abnormal astrocyte morphology / MGI
decreased susceptibility to hepatic steatosis / MGI
abnormal bone marrow morphology / MGI
abnormal erythrocyte morphology / MGI
increased mean platelet volume / MGI
reticulocytosis / MGI
anisocytosis / MGI
decreased circulating insulin level / MGI
microcytic anemia / MGI
microcytosis / MGI
decreased hemoglobin content / MGI
decreased erythrocyte cell number / MGI
increased insulin sensitivity / MGI
increased erythroid progenitor cell number / MGI
loss of hippocampal neurons / MGI
astrocytosis / MGI
abnormal erythrocyte osmotic lysis / MGI
decreased circulating iron level / MGI
abnormal neuronal precursor proliferation / MGI
increased spleen weight / MGI
increased circulating bilirubin level / MGI
renal/urinary system phenotype / MGI
homeostasis/metabolism phenotype / MGI
abnormal axon morphology / MGI
decreased mean corpuscular hemoglobin / MGI
decreased mean corpuscular hemoglobin concentration / MGI
decreased susceptibility to diet-induced obesity / MGI
decreased circulating leptin level / MGI
abnormal inferior vena cava morphology / MGI
optic nerve hypoplasia / MGI
brain vacuoles / MGI
abnormal dendrite morphology / MGI
increased spleen red pulp amount / MGI
slow postnatal weight gain / MGI
decreased circulating interleukin-6 level / MGI
increased spleen iron level / MGI
decreased cerebellar granule cell number / MGI
decreased neuron number / MGI
decreased epididymal fat pad weight / MGI
decreased liver triglyceride level / MGI
decreased oligodendrocyte number / MGI
abnormal neuron differentiation / MGI
increased red blood cell distribution width / MGI
stomatocytosis / MGI
abnormal hepatocyte physiology / MGI
decreased circulating magnesium level / MGI
acanthocytosis / MGI
postnatal lethality, complete penetrance / MGI
increased erythroblast number / MGI
increased circulating erythropoietin level / MGI
increased circulating ferritin level / MGI
abnormal reticulocyte cell number / MGI
decreased food intake / MGI
abnormal ductus venosus morphology / MGI
cerebellum atrophy / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.;DiTommaso Tia, Jones Lynelle K, Cottle Denny L, null null, Gerdin Anna-Karin, Vancollie Valerie E, Watt Fiona M, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, Sundberg John P, White Jacqueline K, Smyth Ian M, ;2014;PLoS genetics;10;e1004705; 25340873

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Prkab1tm1a(KOMP)Wtsi/WtsiPh