C57BL/6N-Atm1Brd Del(10Map3k5-Map7)2Kcl Rhox13tm1a(KOMP)Wtsi/WtsiH[cc]
| Status | Available to order |
| EMMA ID | EM:13795 |
| International strain name | C57BL/6N-Atm1Brd Del(10Map3k5-Map7)2Kcl Rhox13tm1a(KOMP)Wtsi/WtsiH[cc] |
| Alternative name | Rhyme, Del(10Map3k5-Map7)2Kcl |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rhox13tm1a(KOMP)Wtsi, Del(10Map3k5-Map7)2Kcl |
| Gene/Transgene symbol | Rhox13, Del(10Map3k5-Map7)2Kcl |
Information from provider
| Provider | Karen Steel |
| Provider affiliation | King |
| Genetic information | The causative mutation is a 36.7kb deletion, g.10:20116294_20153024del, which includes the last 9 exons of Map3k5 and the first exon of Map7 (Del(10Map3k5-Map7)2Kcl or Rhyme). It arose in a colony carrying the targeted mutation Rhox13tm1a(KOMP)Wtsi, and this allele may still exist in the background. |
| Phenotypic information | Homozygous:Homozygotes for the Rhyme deletion had raised thresholds for detecting responses to sounds at high frequencies at 4 weeks old, and the hearing loss progressed with age. The males are sterile.Heterozygous:No obvious defect. |
| Breeding history | The Rhyme mutation arose spontaneously in a colony carrying the targeted mutation Rhox13tm1a(KOMP)Wtsi, and this allele may still exist in the background. It was maintained within a closed colony on a C57BL/6N background for several years by heterozygous intercrosses or heterozygous males mated with homozygous females. |
| References |
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| Homozygous fertile | females only |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Identification and characterisation of spontaneous mutations causing deafness from a targeted knockout programme.;Lewis Morag A, Ingham Neil J, Chen Jing, Pearson Selina, Di Domenico Francesca, Rekhi Sohinder, Allen Rochelle, Drake Matthew, Willaert Annelore, Rook Victoria, Pass Johanna, Keane Thomas, Adams David J, Tucker Abigail S, White Jacqueline K, Steel Karen P, ;2022;BMC biology;20;67; 35296311