C57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat/Biat
| Status | Available to order |
| EMMA ID | EM:13610 |
| Citation information | RRID:IMSR_EM:13610 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat/Biat |
| Alternative name | C57BL/6N-Tg(Cxcl13-Cre/Tdtomato)723Biat |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Cxcl13-cre,-tdTomato)723Biat |
| Gene/Transgene symbol | Tg(Cxcl13-cre,-tdTomato)723Biat |
Information from provider
| Provider | Burkhard Ludewig |
| Provider affiliation | Institute of Immunobiology, Kantonsspital St.Gallen |
| Genetic information | C57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat mice were generated using BAC RP23-57A17 (Invitrogen). Transgene integration was achieved using Red/ET recombination technologies (Gene bridges) according to the manufacturer’s protocol and as previously described (Sparwasser et al., PMID:14755803). The coding sequence for the Cre-recombinase and the reporter tdTomato, separated by an IRES sequence and including a stop codon, was inserted into Cxcl13 exon 1 using the endogenous ATG start codon. Proper transgene integration was assessed by Southern blot analysis and mice were genotyped using the following primers: forward 5'-TCTCTGCCCAGAGTCATCCT-3', reverse 5'-ATGCTCCTGTCTGTGTGCAG-3'. |
| Phenotypic information | Homozygous:Mice are kept heterozygous in order to avoid germline transmission.Heterozygous:Cre-recombinase and tdTomato are expressed under the control of the Cxcl13 promoter. |
| Breeding history | Mice are bred on C57BL/6 for N>10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Literature references
- Remodeling of light and dark zone follicular dendritic cells governs germinal center responses.;Pikor Natalia B, Mörbe Urs, Lütge Mechthild, Gil-Cruz Cristina, Perez-Shibayama Christian, Novkovic Mario, Cheng Hung-Wei, Nombela-Arrieta César, Nagasawa Takashi, Linterman Michelle A, Onder Lucas, Ludewig Burkhard, ;2020;Nature immunology;21;649-659; 32424359
- Lymphatic Endothelial Cells Control Initiation of Lymph Node Organogenesis.;Onder Lucas, Mörbe Urs, Pikor Natalia, Novkovic Mario, Cheng Hung-Wei, Hehlgans Thomas, Pfeffer Klaus, Becher Burkhard, Waisman Ari, Rülicke Thomas, Gommerman Jennifer, Mueller Christopher G, Sawa Shinichiro, Scandella Elke, Ludewig Burkhard, ;2017;Immunity;47;80-92.e4; 28709801