C57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat/Biat

Status

Available to order

EMMA IDEM:13610
International strain nameC57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat/Biat
Alternative nameC57BL/6N-Tg(Cxcl13-Cre/Tdtomato)723Biat
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Cxcl13-cre,-tdTomato)723Biat
Gene/Transgene symbolTg(Cxcl13-cre,-tdTomato)723Biat

Information from provider

ProviderBurkhard Ludewig
Provider affiliationInstitute of Immunobiology, Kantonsspital St.Gallen
Genetic informationC57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat mice were generated using BAC RP23-57A17 (Invitrogen). Transgene integration was achieved using Red/ET recombination technologies (Gene bridges) according to the manufacturer’s protocol and as previously described (Sparwasser et al., PMID:14755803). The coding sequence for the Cre-recombinase and the reporter tdTomato, separated by an IRES sequence and including a stop codon, was inserted into Cxcl13 exon 1 using the endogenous ATG start codon. Proper transgene integration was assessed by Southern blot analysis and mice were genotyped using the following primers: forward 5'-TCTCTGCCCAGAGTCATCCT-3', reverse 5'-ATGCTCCTGTCTGTGTGCAG-3'.
Phenotypic informationHomozygous:
Mice are kept heterozygous in order to avoid germline transmission.

Heterozygous:
Cre-recombinase and tdTomato are expressed under the control of the Cxcl13 promoter.
Breeding historyMice are bred on C57BL/6 for N>10 generations.
References
  • Remodeling of light and dark zone follicular dendritic cells governs germinal center responses.;Pikor Natalia B, Mörbe Urs, Lütge Mechthild, Gil-Cruz Cristina, Perez-Shibayama Christian, Novkovic Mario, Cheng Hung-Wei, Nombela-Arrieta César, Nagasawa Takashi, Linterman Michelle A, Onder Lucas, Ludewig Burkhard, ;2020;Nature immunology;21;649-659; 32424359
  • Lymphatic Endothelial Cells Control Initiation of Lymph Node Organogenesis.;Onder Lucas, Mörbe Urs, Pikor Natalia, Novkovic Mario, Cheng Hung-Wei, Hehlgans Thomas, Pfeffer Klaus, Becher Burkhard, Waisman Ari, Rülicke Thomas, Gommerman Jennifer, Mueller Christopher G, Sawa Shinichiro, Scandella Elke, Ludewig Burkhard, ;2017;Immunity;47;80-92.e4; 28709801
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria

Literature references

  • Remodeling of light and dark zone follicular dendritic cells governs germinal center responses.;Pikor Natalia B, Mörbe Urs, Lütge Mechthild, Gil-Cruz Cristina, Perez-Shibayama Christian, Novkovic Mario, Cheng Hung-Wei, Nombela-Arrieta César, Nagasawa Takashi, Linterman Michelle A, Onder Lucas, Ludewig Burkhard, ;2020;Nature immunology;21;649-659; 32424359
  • Lymphatic Endothelial Cells Control Initiation of Lymph Node Organogenesis.;Onder Lucas, Mörbe Urs, Pikor Natalia, Novkovic Mario, Cheng Hung-Wei, Hehlgans Thomas, Pfeffer Klaus, Becher Burkhard, Waisman Ari, Rülicke Thomas, Gommerman Jennifer, Mueller Christopher G, Sawa Shinichiro, Scandella Elke, Ludewig Burkhard, ;2017;Immunity;47;80-92.e4; 28709801

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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