C57BL/6N-Cybatm1c(EUCOMM)Wtsi/WtsiKieg

Status

Available to order

EMMA IDEM:13539
International strain nameC57BL/6N-Cybatm1c(EUCOMM)Wtsi/WtsiKieg
Alternative nameEPD0372_5_B08
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolCybatm1c(EUCOMM)Wtsi
Gene/Transgene symbolCyba
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis line originates from EUCOMM ES clone EPD0372_5_B08, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivingheterozygous C57BL/6N

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • impaired righting response / IMPC
  • stereotypic behavior / IMPC
  • decreased blood urea nitrogen level / IMPC
  • persistence of hyaloid vascular system / IMPC
  • decreased circulating amylase level / IMPC
  • increased lean body mass / IMPC
  • cataract / IMPC
  • abnormal gait / IMPC
  • increased mean platelet volume / IMPC
  • trunk curl / IMPC
  • increased leukocyte cell number / IMPC
  • abnormal lens morphology / IMPC
MGI phenotypes (gene matching)
  • impaired swimming / MGI
  • impaired balance / MGI
  • increased inflammatory response / MGI
  • lung inflammation / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal neutrophil physiology / MGI
  • absent otoliths / MGI
  • absent linear vestibular evoked potential / MGI
  • sepsis / MGI
  • head tilt / MGI
  • hearing/vestibular/ear phenotype / MGI
  • abnormal intestinal goblet cell physiology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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