C57BL/6N-Minar2tm1b(KOMP)Wtsi/WtsiKieg

Status

Available to order

EMMA IDEM:13536
International strain nameC57BL/6N-Minar2tm1b(KOMP)Wtsi/WtsiKieg
Alternative nameEPD0770_5_F06
Strain typeTargeted Mutant Strains
Allele/Transgene symbolMinar2tm1b(KOMP)Wtsi
Gene/Transgene symbolMinar2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0770_5_F06. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on KOMP final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
Breeding historyCurrent information may be viewed at the a href="http://www.sanger.ac.uk/mouseportal/search?query=A730017C20Rik"Sanger mouse portal/a: Viability at weaning, Fertility, General Observations
ReferencesNone available

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivingheterozygous C57BL/6N

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased bone mineral density / IMPC
  • decreased circulating phosphate level / IMPC
  • decreased body length / IMPC
  • abnormal bone structure / IMPC
  • decreased lean body mass / IMPC
  • abnormal auditory brainstem response / IMPC
  • decreased circulating glucose level / IMPC
  • absent pinna reflex / IMPC
  • increased total body fat amount / IMPC
  • decreased bone mineral content / IMPC
IMPC phenotypes (gene matching)
  • increased total body fat amount / IMPC
  • abnormal bone structure / IMPC
  • decreased circulating glucose level / IMPC
  • decreased bone mineral content / IMPC
  • absent pinna reflex / IMPC
  • abnormal auditory brainstem response / IMPC
  • decreased lean body mass / IMPC
  • decreased bone mineral density / IMPC
  • decreased body length / IMPC
  • decreased circulating phosphate level / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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