C57BL/6N-Adcy9^{tm1b(EUCOMM)Wtsi}/WtsiKieg

Status	Available to order
EMMA ID	EM:13535
International strain name	C57BL/6N-Adcy9^{tm1b(EUCOMM)Wtsi}/WtsiKieg
Alternative name	EPD0382_6_B06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Adcy9^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Adcy9
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0382_6_B06. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden
Animals used for archiving	heterozygous C57BL/6N

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating insulin level / IMPC
decreased circulating chloride level / IMPC
preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

decreased circulating chloride level / IMPC
preweaning lethality, incomplete penetrance / IMPC
increased circulating insulin level / IMPC

MGI phenotypes (allele matching)

abnormal small intestine placement / MGI
enlarged liver sinusoidal spaces / MGI
small superior cervical ganglion / MGI
abnormal Mullerian duct morphology / MGI
liver hemorrhage / MGI
absent stapedial artery / MGI
abnormal temporal bone morphology / MGI
abnormal right lung accessory lobe morphology / MGI
perimembraneous ventricular septal defect / MGI
dual inferior vena cava / MGI
absent segment of posterior cerebral artery / MGI
retropleural edema / MGI
abnormal Mullerian duct topology / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve morphology / MGI
blood in lymph vessels / MGI
abnormal umbilical vein topology / MGI
abnormal vertebral artery topology / MGI
additional anastomosis between intracranial vertebral arteries / MGI

MGI phenotypes (gene matching)

abnormal small intestine placement / MGI
enlarged liver sinusoidal spaces / MGI
small superior cervical ganglion / MGI
no abnormal phenotype detected / MGI
abnormal Mullerian duct morphology / MGI
liver hemorrhage / MGI
absent stapedial artery / MGI
abnormal temporal bone morphology / MGI
increased IgG1 level / MGI
abnormal right lung accessory lobe morphology / MGI
perimembraneous ventricular septal defect / MGI
dual inferior vena cava / MGI
absent segment of posterior cerebral artery / MGI
retropleural edema / MGI
abnormal Mullerian duct topology / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve morphology / MGI
blood in lymph vessels / MGI
abnormal umbilical vein topology / MGI
abnormal vertebral artery topology / MGI
additional anastomosis between intracranial vertebral arteries / MGI
retrolental blood / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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