C57BL/6NCrl-Mettl5^{em1(IMPC)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:13474
International strain name	C57BL/6NCrl-Mettl5^{em1(IMPC)Hmgu}/Ieg
Alternative name
Strain type	Endonuclease-mediated
Allele/Transgene symbol	Mettl5^{em1(IMPC)Hmgu}
Gene/Transgene symbol	Mettl5

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from CRISPR zygote microinjection. For further details see the project page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	The rRNA m⁶A methyltransferase METTL5 is involved in pluripotency and developmental programs.;Ignatova Valentina V, Stolz Paul, Kaiser Steffen, Gustafsson Tobias H, Lastres Palma Rico, Sanz-Moreno Adrián, Cho Yi-Li, Amarie Oana V, Aguilar-Pimentel Antonio, Klein-Rodewald Tanja, Calzada-Wack Julia, Becker Lore, Marschall Susan, Kraiger Markus, Garrett Lillian, Seisenberger Claudia, Hölter Sabine M, Borland Kayla, Van De Logt Erik, Jansen Pascal W T C, Baltissen Marijke P, Valenta Magdalena, Vermeulen Michiel, Wurst Wolfgang, Gailus-Durner Valerie, Fuchs Helmut, Hrabe de Angelis Martin, Rando Oliver J, Kellner Stefanie M, Bultmann Sebastian, Schneider Robert, ;2020;Genes & development;34;715-729; 32217665

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6N

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive primary microcephaly / Orphanet_2512

IMPC phenotypes (allele matching)

decreased hematocrit / IMPC
abnormal head morphology / IMPC
abnormal cranium morphology / IMPC
decreased body length / IMPC
persistence of hyaloid vascular system / IMPC
abnormal lens morphology / IMPC
cataract / IMPC
abnormal cornea morphology / IMPC
abnormal iris morphology / IMPC
abnormal retina morphology / IMPC
decreased anxiety-related response / IMPC
hyperactivity / IMPC
decreased locomotor activity / IMPC
increased hematocrit / IMPC
abnormal vitreous body morphology / IMPC
decreased vertical activity / IMPC
abnormal retina vasculature morphology / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
iris synechia / IMPC
abnormal auditory brainstem response / IMPC
abnormal behavior / IMPC
increased spleen weight / IMPC
decreased blood urea nitrogen level / IMPC
abnormal optic disk morphology / IMPC
increased grip strength / IMPC
preweaning lethality, incomplete penetrance / IMPC
decreased thigmotaxis / IMPC

IMPC phenotypes (gene matching)

abnormal cornea morphology / IMPC
abnormal head morphology / IMPC
persistence of hyaloid vascular system / IMPC
abnormal iris morphology / IMPC
cataract / IMPC
abnormal auditory brainstem response / IMPC
decreased hemoglobin content / IMPC
decreased thigmotaxis / IMPC
decreased erythrocyte cell number / IMPC
preweaning lethality, incomplete penetrance / IMPC
abnormal cranium morphology / IMPC
abnormal vitreous body morphology / IMPC
iris synechia / IMPC
hyperactivity / IMPC
decreased vertical activity / IMPC
abnormal optic disk morphology / IMPC
abnormal lens morphology / IMPC
decreased locomotor activity / IMPC
abnormal retina morphology / IMPC
decreased body length / IMPC
abnormal behavior / IMPC
increased grip strength / IMPC
abnormal retina vasculature morphology / IMPC
increased spleen weight / IMPC
decreased anxiety-related response / IMPC
increased hematocrit / IMPC
decreased blood urea nitrogen level / IMPC
decreased hematocrit / IMPC

Literature references

The rRNA m⁶A methyltransferase METTL5 is involved in pluripotency and developmental programs.;Ignatova Valentina V, Stolz Paul, Kaiser Steffen, Gustafsson Tobias H, Lastres Palma Rico, Sanz-Moreno Adrián, Cho Yi-Li, Amarie Oana V, Aguilar-Pimentel Antonio, Klein-Rodewald Tanja, Calzada-Wack Julia, Becker Lore, Marschall Susan, Kraiger Markus, Garrett Lillian, Seisenberger Claudia, Hölter Sabine M, Borland Kayla, Van De Logt Erik, Jansen Pascal W T C, Baltissen Marijke P, Valenta Magdalena, Vermeulen Michiel, Wurst Wolfgang, Gailus-Durner Valerie, Fuchs Helmut, Hrabe de Angelis Martin, Rando Oliver J, Kellner Stefanie M, Bultmann Sebastian, Schneider Robert, ;2020;Genes & development;34;715-729; 32217665

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NCrl-Mettl5em1(IMPC)Hmgu/Ieg