B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes Tg(tetO-Cpt1a,-EGFP)/Cnbc

Status	Available to order
EMMA ID	EM:13348
International strain name	B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes Tg(tetO-Cpt1a,-EGFP)/Cnbc
Alternative name	Pax8-rtTAtg/0:tetO-Cpt1atg/0 (Pax8-CPT1A)
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(tetO-Cpt1a), Tg(Pax8-rtTA2S*M2)1Koes
Gene/Transgene symbol	Tg(tetO-Cpt1a,-EGFP), Tg(Pax8-rtTA2S*M2)1Koes

Information from provider

Provider	Santiago Lamas
Provider affiliation	Procesos Fisiológicos y Patológicos, Centro de Biología Molecular Severo Ochoa
Genetic information	Pax8-rtTA cassette (Chr 8): expression of rtTA in renal proximal epithelial cells. TRE-CPT1A-P2A-GFP cassette (Col1a1 locus): CPT1A and GFP coding sequence under the control of the TRE element. P2A allows the protein excision among CPT1A and GFP.
Phenotypic information	Homozygous: Phenotypically undistinguishable from wild-type mice. No anatomical anomalies, physiological variations, behavioural abnormalities or food uptake alterations. Heterozygous: No phenotypic alterations. Mice present a CPT1A overexpression and enhanced fatty acid oxidation in the tubular cells in the kidney.
Breeding history	Pax8-rtTA mice (C57BL/6J) (JAX number 007176) were crossed with the newly generated TRE-CPT1A mice (B6N;129/Sv background). Resulting mice were backcrossed to C57BL/6J mice for at least 5 generations to generate this congenic strain.
References	Renal tubule Cpt1a overexpression protects from kidney fibrosis by restoring mitochondrial homeostasis.;Miguel Verónica, Tituaña Jessica, Herrero J Ignacio, Herrero Laura, Serra Dolors, Cuevas Paula, Barbas Coral, Puyol Diego Rodríguez, Márquez-Expósito Laura, Ruiz-Ortega Marta, Castillo Carolina, Sheng Xin, Susztak Katalin, Ruiz-Canela Miguel, Salas-Salvadó Jordi, González Miguel A Martínez, Ortega Sagrario, Ramos Ricardo, Lamas Santiago, ;2021;The Journal of clinical investigation;131;; 33465052
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6J

Literature references

Renal tubule Cpt1a overexpression protects from kidney fibrosis by restoring mitochondrial homeostasis.;Miguel Verónica, Tituaña Jessica, Herrero J Ignacio, Herrero Laura, Serra Dolors, Cuevas Paula, Barbas Coral, Puyol Diego Rodríguez, Márquez-Expósito Laura, Ruiz-Ortega Marta, Castillo Carolina, Sheng Xin, Susztak Katalin, Ruiz-Canela Miguel, Salas-Salvadó Jordi, González Miguel A Martínez, Ortega Sagrario, Ramos Ricardo, Lamas Santiago, ;2021;The Journal of clinical investigation;131;; 33465052

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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