B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes Tg(tetO-Cpt1a,-EGFP)/Cnbc
| Status | Available to order |
| EMMA ID | EM:13348 |
| Citation information | RRID:IMSR_EM:13348 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes Tg(tetO-Cpt1a,-EGFP)/Cnbc |
| Alternative name | Pax8-rtTAtg/0:tetO-Cpt1atg/0 (Pax8-CPT1A) |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-Cpt1a), Tg(Pax8-rtTA2S*M2)1Koes |
| Gene/Transgene symbol | Tg(tetO-Cpt1a,-EGFP), Tg(Pax8-rtTA2S*M2)1Koes |
Information from provider
| Provider | Santiago Lamas |
| Provider affiliation | Procesos Fisiológicos y Patológicos, Centro de Biología Molecular Severo Ochoa |
| Genetic information | Pax8-rtTA cassette (Chr 8): expression of rtTA in renal proximal epithelial cells. TRE-CPT1A-P2A-GFP cassette (Col1a1 locus): CPT1A and GFP coding sequence under the control of the TRE element. P2A allows the protein excision among CPT1A and GFP. |
| Phenotypic information | Homozygous:Phenotypically undistinguishable from wild-type mice. No anatomical anomalies, physiological variations, behavioural abnormalities or food uptake alterations.Heterozygous:No phenotypic alterations. Mice present a CPT1A overexpression and enhanced fatty acid oxidation in the tubular cells in the kidney. |
| Breeding history | Pax8-rtTA mice (C57BL/6J) (JAX number 007176) were crossed with the newly generated TRE-CPT1A mice (B6N;129/Sv background). Resulting mice were backcrossed to C57BL/6J mice for at least 5 generations to generate this congenic strain. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- Renal tubule Cpt1a overexpression protects from kidney fibrosis by restoring mitochondrial homeostasis.;Miguel Verónica, Tituaña Jessica, Herrero J Ignacio, Herrero Laura, Serra Dolors, Cuevas Paula, Barbas Coral, Puyol Diego Rodríguez, Márquez-Expósito Laura, Ruiz-Ortega Marta, Castillo Carolina, Sheng Xin, Susztak Katalin, Ruiz-Canela Miguel, Salas-Salvadó Jordi, González Miguel A Martínez, Ortega Sagrario, Ramos Ricardo, Lamas Santiago, ;2021;The Journal of clinical investigation;131;; 33465052