IMPC phenotypes (gene matching)
Wistar Kyoto rat NOX4KO
| Status | Available to order |
| EMMA ID | EM:13226 |
| International strain name | Wistar Kyoto rat NOX4KO |
| Alternative name | NOX4KO rat |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Deletion in the NOX4 gene |
| Gene/Transgene symbol | Nox4 |
Information from provider
| Provider | Harald Schmidt |
| Provider affiliation | Pharmacology & Personalised Therapy, Maastricht University |
| Genetic information | Deletion in the NADPH oxidase 4 gene using the CompoZr Zinc Finger Nuclease technology. The E14-15 domain (2.3-2.4 Kb) of the Nox4 gene was removed in a WKY background rat. Zinc-Finger Nucleases (ZFNs) were coupled with a FOK1 endonuclease and designed to recognize and cleave the specific NOX4 sequence producing sequence-specific double-strand breaks that are repaired by error-prone non-homologous end joining (NHEJ) or high-fidelity homologous recombination (HR). The Nox4 KO rat was generated by introducing variable genomic deletions that result in a frameshift within the open reading frame. The frameshifts often result in the introduction of a premature stop codon. When the premature stop codon occurs before the last exon, the transcript is likely degraded via nonsense mediated decay pathway and little or no protein is expressed. Resulting animals were screened for mutations and complete genomic sequencing was performed. |
| Phenotypic information | Homozygous:These rats do not show any specific phenotype related to the mutation.Heterozygous:Same as above. No specific phenotype detected. |
| Breeding history | These animals have been breeding at Maastricht University (The Netherlands) since they were generated. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous 0, homozygous 0 |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- NOX4-dependent neuronal autotoxicity and BBB breakdown explain the superior sensitivity of the brain to ischemic damage.;Casas Ana I, Geuss Eva, Kleikers Pamela W M, Mencl Stine, Herrmann Alexander M, Buendia Izaskun, Egea Javier, Meuth Sven G, Lopez Manuela G, Kleinschnitz Christoph, Schmidt Harald H H W, ;2017;Proceedings of the National Academy of Sciences of the United States of America;114;12315-12320; 29087944