B6;Cg-Pmeltm1.1(cre/ERT2)Jkn/H

Status

Available to order

EMMA IDEM:13225
International strain nameB6;Cg-Pmeltm1.1(cre/ERT2)Jkn/H
Alternative namePmel-CMN
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolPmeltm1.1(cre/ERT2)Jkn
Gene/Transgene symbolPmel

Information from provider

ProviderRichard Mort
Provider affiliationBiomedical and Life Sciences, Lancaster University
Additional ownerFrank Constantini's group developed the R26YFPR allele but widely available.
Genetic informationPmel-CMN mice carry a knock-in cassette replacing the STOP codon of the Pmel gene with creERT2-2a-marcks-mKate-2a-H2BCerulean.
Phenotypic informationHomozygous:
No phenotype.

Heterozygous:
No phenotype.
Breeding historyPmel-CMN generated in E14 129P2/OlaHsd ES cells. Outcrossed to C57BL/6.
References
  • Genetically engineered multicistronic allele of Pmel yielding highly specific CreERT2-mediated recombination in the melanocyte lineage.;Wilkinson Emma L, Brennan Louise C, Harrison Olivia J, Crane-Smith Zoe, Gautier Philippe, Keighren Margaret A, Budd Peter, Swaminathan Karthic, Machesky Laura M, Allinson Sarah L, Jackson Ian J, Mort Richard L, ;2023;Pigment cell & melanoma research;36;71-77; 36412082
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased circulating bilirubin level / IMPC
  • increased startle reflex / IMPC
  • decreased total body fat amount / IMPC
MGI phenotypes (gene matching)
  • diluted coat color / MGI
  • irregular coat pigmentation / MGI
  • pigmentation phenotype / MGI
  • no phenotypic analysis / MGI
  • abnormal melanosome morphology / MGI
  • homeostasis/metabolism phenotype / MGI
  • hematopoietic system phenotype / MGI
  • reduced hair shaft melanin granule number / MGI
  • abnormal tail hair pigmentation / MGI
  • abnormal retinal melanin granule morphology / MGI
  • abnormal choroid melanin granule morphology / MGI

Literature references

  • Genetically engineered multicistronic allele of Pmel yielding highly specific CreERT2-mediated recombination in the melanocyte lineage.;Wilkinson Emma L, Brennan Louise C, Harrison Olivia J, Crane-Smith Zoe, Gautier Philippe, Keighren Margaret A, Budd Peter, Swaminathan Karthic, Machesky Laura M, Allinson Sarah L, Jackson Ian J, Mort Richard L, ;2023;Pigment cell & melanoma research;36;71-77; 36412082

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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