C57BL/6N-Acbd5tm1b(EUCOMM)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:13214
International strain nameC57BL/6N-Acbd5tm1b(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0633_5_D12
Strain typeTargeted Mutant Strains
Allele/Transgene symbolAcbd5tm1b(EUCOMM)Wtsi
Gene/Transgene symbolAcbd5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0633_5_D12. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased bone mineral density / IMPC
  • decreased circulating calcium level / IMPC
  • increased body length / IMPC
  • abnormal gait / IMPC
  • limb grasping / IMPC
  • increased mean platelet volume / IMPC
  • decreased circulating triglyceride level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • thrombocytopenia / IMPC
  • increased lean body mass / IMPC
  • improved glucose tolerance / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased circulating creatinine level / IMPC
  • increased blood urea nitrogen level / IMPC
  • decreased circulating total protein level / IMPC
  • decreased total body fat amount / IMPC
  • increased bone mineral content / IMPC
  • decreased bone trabecula number / IMPC
  • increased bone trabecular spacing / IMPC
IMPC phenotypes (gene matching)
  • limb grasping / IMPC
  • increased lean body mass / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased blood urea nitrogen level / IMPC
  • increased body length / IMPC
  • increased bone trabecular spacing / IMPC
  • decreased total body fat amount / IMPC
  • increased mean platelet volume / IMPC
  • decreased circulating total protein level / IMPC
  • abnormal gait / IMPC
  • increased bone mineral density / IMPC
  • improved glucose tolerance / IMPC
  • decreased bone trabecula number / IMPC
  • increased bone mineral content / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased circulating triglyceride level / IMPC
  • decreased circulating calcium level / IMPC
  • increased circulating creatinine level / IMPC
  • thrombocytopenia / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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