C57BL/6NCrl-Rnf168tm2b(EUCOMM)Hmgu/Ph

Status

Under development - register interest

EMMA IDEM:13197
International strain nameC57BL/6NCrl-Rnf168tm2b(EUCOMM)Hmgu/Ph
Alternative nameHEPD0798_7_B10
Strain typeTargeted Mutant Strains
Allele/Transgene symbolRnf168tm2b(EUCOMM)Hmgu
Gene/Transgene symbolRnf168
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Institute of Molecular Genetics
Provider affiliationDepartment of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0798_7_B10. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased hematocrit / IMPC
  • abnormal heart morphology / IMPC
  • enlarged heart / IMPC
  • abnormal head morphology / IMPC
  • abnormal cranium morphology / IMPC
  • abnormal spleen morphology / IMPC
  • abnormal thymus morphology / IMPC
  • enlarged thymus / IMPC
  • decreased body weight / IMPC
  • anophthalmia / IMPC
  • microphthalmia / IMPC
  • fused cornea and lens / IMPC
  • abnormal cornea morphology / IMPC
  • increased circulating phosphate level / IMPC
  • abnormal skin morphology / IMPC
  • abnormal eye morphology / IMPC
  • increased mean platelet volume / IMPC
  • decreased hemoglobin content / IMPC
  • decreased erythrocyte cell number / IMPC
  • increased circulating alanine transaminase level / IMPC
  • abnormal spine curvature / IMPC
  • vertebral fusion / IMPC
  • increased eosinophil cell number / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • increased grip strength / IMPC
  • increased circulating creatine kinase level / IMPC
  • increased circulating lactate dehydrogenase level / IMPC
IMPC phenotypes (gene matching)
  • increased mean platelet volume / IMPC
  • abnormal spleen morphology / IMPC
  • increased circulating creatine kinase level / IMPC
  • vertebral fusion / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • decreased hematocrit / IMPC
  • abnormal cornea morphology / IMPC
  • abnormal coat/hair pigmentation / IMPC
  • increased circulating alanine transaminase level / IMPC
  • increased grip strength / IMPC
  • abnormal snout morphology / IMPC
  • abnormal cranium morphology / IMPC
  • fused cornea and lens / IMPC
  • abnormal thymus morphology / IMPC
  • abnormal head morphology / IMPC
  • abnormal spine curvature / IMPC
  • abnormal eye morphology / IMPC
  • enlarged thymus / IMPC
  • abnormal heart morphology / IMPC
  • increased circulating lactate dehydrogenase level / IMPC
  • microphthalmia / IMPC
  • anophthalmia / IMPC
  • decreased spleen weight / IMPC
  • corneal opacity / IMPC
  • abnormal skin morphology / IMPC
  • enlarged heart / IMPC
  • increased circulating phosphate level / IMPC
  • increased eosinophil cell number / IMPC
  • decreased body weight / IMPC
  • decreased hemoglobin content / IMPC
  • narrow eye opening / IMPC
  • decreased erythrocyte cell number / IMPC

Information on how we integrate external resources can be found here

Register interest

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

    Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

    * In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

    More details on pricing and delivery times

    Practical information

    Example health report
    (Current health report will be provided later)

    Material Transfer Agreement (MTA)
    Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

    EMMA conditions
    Legally binding conditions for the transfer

    Other EMMA strains

    Not found what you were looking for? Search here for other strains available from EMMA.


    Search
    INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).