C57BL/6NTac-Map3k1^{tm1a(KOMP)Wtsi}/WtsiFlmg

Status	Available to order
EMMA ID	EM:13176
International strain name	C57BL/6NTac-Map3k1^{tm1a(KOMP)Wtsi}/WtsiFlmg
Alternative name	EPD0287_3_A11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Map3k1^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Map3k1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0287_3_A11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

46,XY complete gonadal dysgenesis / Orphanet_242
46,XY partial gonadal dysgenesis / Orphanet_251510

IMPC phenotypes (allele matching)

abnormal retina morphology / IMPC
increased circulating free fatty acids level / IMPC
increased lean body mass / IMPC
increased red blood cell distribution width / IMPC
decreased circulating fructosamine level / IMPC
increased bone mineral content / IMPC
preweaning lethality, incomplete penetrance / IMPC
decreased fasting circulating glucose level / IMPC

IMPC phenotypes (gene matching)

increased red blood cell distribution width / IMPC
preweaning lethality, incomplete penetrance / IMPC
abnormal retina morphology / IMPC
abnormal startle reflex / IMPC
increased lean body mass / IMPC
decreased fasting circulating glucose level / IMPC
increased bone mineral content / IMPC
increased circulating free fatty acids level / IMPC
decreased circulating fructosamine level / IMPC

MGI phenotypes (gene matching)

organ of Corti degeneration / MGI
microphthalmia / MGI
eyelids open at birth / MGI
corneal opacity / MGI
abnormal eyelid morphology / MGI
abnormal pinna reflex / MGI
heart inflammation / MGI
abnormal eye morphology / MGI
abnormal keratinocyte morphology / MGI
exophthalmos / MGI
early eyelid opening / MGI
increased cardiomyocyte apoptosis / MGI
abnormal retinal layer morphology / MGI
abnormal heart left ventricle morphology / MGI
decreased cochlear inner hair cell number / MGI
increased cochlear outer hair cell number / MGI
cochlear outer hair cell degeneration / MGI
increased response of heart to induced stress / MGI
abnormal vascular wound healing / MGI
abnormal eye physiology / MGI
hearing/vestibular/ear phenotype / MGI
immune system phenotype / MGI
abnormal vascular smooth muscle physiology / MGI
decreased ventricle muscle contractility / MGI
congestive heart failure / MGI
abnormal eyelid fusion / MGI
abnormal eyelid development / MGI
increased sensitivity to induced morbidity/mortality / MGI
impaired fibroblast cell migration / MGI
increased or absent threshold for auditory brainstem response / MGI
abnormal eyelid margin morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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