129;MF1-Igf1tm1Arge/Cnbc
| Status | Available to order |
| EMMA ID | EM:13159 |
| International strain name | 129;MF1-Igf1tm1Arge/Cnbc |
| Alternative name | Igf1tm1Arge_129Sv-MF1 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Igf1tm1Arge |
| Gene/Transgene symbol | Igf1 |
Information from provider
| Provider | Isabel Varela Nieto |
| Provider affiliation | Instituto de Investigaciones Biomédicas "Alberto Sols" |
| Additional owner | Argiris Efstratiadis, MD, PhD, Biomedical Research Foundation of the Academy of Athens (BRFAA) |
| Genetic information | Replacement of sequences encoding part of exon 4 with a neomycin resistance gene. |
| Phenotypic information | Homozygous:Mutant mice lacking the Igf1 gene have severe embryonic and postnatal growth retardation that are coupled to diminished survival and delayed nerve myelination. The homozygous mutant mice maintained on a hybrid background (MF1 x 129S/Sv) present delayed inner ear development and, as a consequence, develop a profound syndromic sensorineural hearing loss with increased hearing thresholds.Heterozygous:The young heterozygous mice present normal hearing and show no evident cellular alterations. |
| Breeding history | The mice were obtained by backcrossing the original line on MF1 x 129S/Sv background (Liu et al., 1993, doi: 10.1016/S0092-8674(05)80084-4), selected because of the increased survival of the homozygous mutant progeny. The original mutant line was firstly backcrossed to the MF1 background at least 10 times, and 2 more times in the last 10 years to the 129S/SvEv background. Wild-type males and females were used. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous 129S/SvEv x MF1 |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Growth delay due to insulin-like growth factor type 1 deficiency / Orphanet_73272
Literature references
- A comparative study of age-related hearing loss in wild type and insulin-like growth factor I deficient mice.;Riquelme Raquel, Cediel Rafael, Contreras Julio, la Rosa Lourdes Rodriguez-de, Murillo-Cuesta Silvia, Hernandez-Sanchez Catalina, Zubeldia Jose M, Cerdan Sebastian, Varela-Nieto Isabel, ;2010;Frontiers in neuroanatomy;4;27; 20661454
- RNA microarray analysis in prenatal mouse cochlea reveals novel IGF-I target genes: implication of MEF2 and FOXM1 transcription factors.;Sanchez-Calderon Hortensia, Rodriguez-de la Rosa Lourdes, Milo Marta, Pichel Jose G, Holley Matthew, Varela-Nieto Isabel, ;2010;PloS one;5;e8699; 20111592
- Delayed inner ear maturation and neuronal loss in postnatal Igf-1-deficient mice.;Camarero G, Avendano C, Fernandez-Moreno C, Villar A, Contreras J, de Pablo F, Pichel J G, Varela-Nieto I, ;2001;The Journal of neuroscience : the official journal of the Society for Neuroscience;21;7630-41; 11567053
- Sensorineural hearing loss in insulin-like growth factor I-null mice: a new model of human deafness.;Cediel R, Riquelme R, Contreras J, Díaz A, Varela-Nieto I, ;2006;The European journal of neuroscience;23;587-90; 16420467
- Mice carrying null mutations of the genes encoding insulin-like growth factor I (Igf-1) and type 1 IGF receptor (Igf1r).;Liu J P, Baker J, Perkins A S, Robertson E J, Efstratiadis A, ;1993;Cell;75;59-72; 8402901